Pressure cryocooling protein crystals

Kim, Chae Un; Gruner, Sol M

Title: Pressure cryocooling protein crystals

Patent · Tue Oct 04 00:00:00 EDT 2011

OSTI ID:1029280

Kim, Chae Un ^[1]; Gruner, Sol M ^[1]

Ithaca, NY

Preparation of cryocooled protein crystal is provided by use of helium pressurizing and cryocooling to obtain cryocooled protein crystal allowing collection of high resolution data and by heavier noble gas (krypton or xenon) binding followed by helium pressurizing and cryocooling to obtain cryocooled protein crystal for collection of high resolution data and SAD phasing simultaneously. The helium pressurizing is carried out on crystal coated to prevent dehydration or on crystal grown in aqueous solution in a capillary.

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Research Organization:: Cornell Univ., Ithaca, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-97ER62443

Assignee:: Cornell Research Foundation, Inc. (Ithaca, NY)

Patent Number(s):: 8,030,449

Application Number:: 11/700,237

OSTI ID:: 1029280

Country of Publication:: United States

Language:: English

References (13)

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Freezing of myoglobin crystals at high pressure Thomanek, U. F.; Parak, F.; Mössbauer, R. L. Acta Crystallographica Section A, Vol. 29, Issue 3 https://doi.org/10.1107/S0567739473000677	journal	May 1973
High-pressure cooling of protein crystals without cryoprotectants Kim, Chae Un; Kapfer, Raphael; Gruner, Sol M. Acta Crystallographica Section D Biological Crystallography, Vol. 61, Issue 7 https://doi.org/10.1107/S090744490500836X	journal	June 2005
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Solution of protein crystallographic structures by high-pressure cryocooling and noble-gas phasing Kim, Chae Un; Hao, Quan; Gruner, Sol M. Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 7 https://doi.org/10.1107/S0907444906014727	journal	June 2006
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