skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Pressure cryocooling protein crystals

Abstract

Preparation of cryocooled protein crystal is provided by use of helium pressurizing and cryocooling to obtain cryocooled protein crystal allowing collection of high resolution data and by heavier noble gas (krypton or xenon) binding followed by helium pressurizing and cryocooling to obtain cryocooled protein crystal for collection of high resolution data and SAD phasing simultaneously. The helium pressurizing is carried out on crystal coated to prevent dehydration or on crystal grown in aqueous solution in a capillary.

Inventors:
 [1];  [1]
  1. Ithaca, NY
Publication Date:
Research Org.:
Cornell Univ., Ithaca, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1029280
Patent Number(s):
8,030,449
Application Number:
11/700,237
Assignee:
Cornell Research Foundation, Inc. (Ithaca, NY)
DOE Contract Number:  
FG02-97ER62443
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Kim, Chae Un, and Gruner, Sol M. Pressure cryocooling protein crystals. United States: N. p., 2011. Web.
Kim, Chae Un, & Gruner, Sol M. Pressure cryocooling protein crystals. United States.
Kim, Chae Un, and Gruner, Sol M. 2011. "Pressure cryocooling protein crystals". United States. https://www.osti.gov/servlets/purl/1029280.
@article{osti_1029280,
title = {Pressure cryocooling protein crystals},
author = {Kim, Chae Un and Gruner, Sol M},
abstractNote = {Preparation of cryocooled protein crystal is provided by use of helium pressurizing and cryocooling to obtain cryocooled protein crystal allowing collection of high resolution data and by heavier noble gas (krypton or xenon) binding followed by helium pressurizing and cryocooling to obtain cryocooled protein crystal for collection of high resolution data and SAD phasing simultaneously. The helium pressurizing is carried out on crystal coated to prevent dehydration or on crystal grown in aqueous solution in a capillary.},
doi = {},
url = {https://www.osti.gov/biblio/1029280}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 04 00:00:00 EDT 2011},
month = {Tue Oct 04 00:00:00 EDT 2011}
}

Works referenced in this record:

Crystal growth in X-ray-transparent plastic tubing: an alternative for high-throughput applications
journal, October 2005


Crystallizing a Protein
book, January 1994


Freezing of myoglobin crystals at high pressure
journal, May 1973


High-pressure cooling of protein crystals without cryoprotectants
journal, June 2005


Protein recognition of macrocycles: Binding of anti-HIV metallocyclams to lysozyme
journal, February 2005


Models of protein crystal growth
journal, June 2001


New Strategies for Protein Crystal Growth
journal, August 1999


The RCK Domain of the KtrAB K+ Transporter: Multiple Conformations of an Octameric Ring
journal, September 2006


Crystallogenesis of biological macromolecules: facts and perspectives
journal, July 1994


Probing Substates in Sperm Whale Myoglobin Using High-Pressure Crystallography
journal, January 2002


Solution of protein crystallographic structures by high-pressure cryocooling and noble-gas phasing
journal, June 2006


MAD phasing with krypton
journal, February 2001