Arsenic speciation using high performance liquid chromatography-inductively coupled plasma-mass spectrometry

Technical Report · Tue Aug 01 04:00:00 EDT 1995

DOI:https://doi.org/10.2172/102125· OSTI ID:102125

Bass, D A; Yaeger, J S; Crain, J S; Kiely, J T; Parish, K J; Gowdy, M J ^[1]; Mohrman, G B ^[2]

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1. Argonne National Lab., IL (United States)
2. Rocky Mountain Arsenal, Commerce City, CO (United States)

A method has been developed by Argonne National Laboratory to identify and quantify As(III), As(V), and organoarsenic compounds in environmental samples. A arsenic species were separated by reversed-phase, ion-pairing, HPLC using a microbore Inertsil-ODS{trademark} column. Only 1 {micro}L of sample was injected on the column, and the mobile phase flow rates were typically on the order of 40 {micro}L/min. The HPLC mobile phase was a mixture of methanol and tetrabutylammonium hydroxide (TBAH), and the column effluent was introduced into an ICP-mass spectrometer using direct injection nebulization. Detection limits of less than 1 pg As (as injected on the column) were easily obtained for each arsenic species. The effect of changes in mobile phase composition and ICP-MS conditions will be described, as well as quality control measures, e.g., the use of surrogates, internal standards, and matrix spikes. Precision and accuracy information will be presented from the analysis of aqueous standards and soil extracts that were spiked with arsenic oxide [As(III)], sodium arsenate [As(V)], dimethylarsinic acid (DMAA), or chlorovinyl arsenious acid (CVAA). The authors believe that these data demonstrate the utility of this technique for the sensitive determination of arsenic species present in water or soil.

• Research Organization: Argonne National Lab., IL (United States)
• Sponsoring Organization: Department of Defense, Washington, DC (United States); USDOE, Washington, DC (United States)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 102125
• Report Number(s): ANL/CMT-ACL/VU--86365; CONF-9506239--1; ON: DE95015641
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
54 ENVIRONMENTAL SCIENCES
ARSENIC
ARSENIC COMPOUNDS
CHEMICAL ANALYSIS
ENVIRONMENTAL MATERIALS
EXPERIMENTAL DATA
EXTRACTION CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
ORGANIC ARSENIC COMPOUNDS
VALENCE