Flow cytometric detection method for DNA samples

Patent · Tue Jul 05 00:00:00 EDT 2011

OSTI ID:1019570

Nasarabadi, Shanavaz ^[1]; Langlois, Richard G ^[1]; Venkateswaran, Kodumudi S ^[2]

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1. Livermore, CA
2. Round Rock, TX

Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-48
• Assignee: Lawrence Livermore National Security, LLC (Livermore, CA)
• Patent Number(s): 7,972,818
• Application Number: US Patent Application 11/454,478
• OSTI ID: 1019570
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES