Physiological and phylogenetic study of microbes from geochemically and hydrogeologically diverse subsurface environments. Interim technical report, November 1, 1991--September 30, 1992

Technical Report · Thu Oct 01 04:00:00 EDT 1992

OSTI ID:10184165

Balkwill, D L; Reeves, R H

An important aspect of our ongoing research effort in the DOE Deep Microbiology Program has been the characterization of certain microorganisms in samples of subsurface materials from various DOE production sites. Recently, it was our responsibility to determine the numbers, diversity, morphological characteristics, and physiological traits (including the potential metabolic capabilities) of the viable, aerobic, chemoheterotrophic micro-organisms in samples that were obtained from the Idaho National Engineering Laboratory (INEL) and the Hanford Reservation (HR) in 1990. It was decided that the physiological characteristics of the INEL and HR subsurface microbial isolates would be analyzed during the current project. We describe here the research on physiological characteristics that has been completed during the eleven-month period covered by the present technical report. Approximately 750 microbial strains were isolated by our laboratory from the INEL and HR samples in 1990 and 1991. During the portion of the current project covered by this interim report, all of these strains were tested for their ability to utilize each of 95 different organic compounds as sole sources of carbon. Identifications are based on how well the carbon utilization patterns of the isolates match those of 659 species and subspecies (i.e., established taxa).

• Research Organization: Florida State Univ., Tallahassee, FL (United States). Dept. of Biological Science
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG05-91ER61159; FG05-90ER61039
• OSTI ID: 10184165
• Report Number(s): DOE/ER/61159--2; ON: DE93001846
• Country of Publication: United States
• Language: English

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BIOLOGICAL EVOLUTION
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