Physiologically anaerobic microorganisms of the deep subsurface. Final performance report, June 1, 1990--August 31, 1993
Anaerobic bacteria were isolated from deep subsurface sediment samples taken at study sites in Idaho (INEL) and Washington (HR) by culturing on dilute and concentrated medium. Morphologically distinct colonies were purified, and their responses to 21 selected physiological tests were determined. Although the number of isolates was small (18 INEL, 27 HR) some general patterns could be determined. Most strains could utilize all the carbon sources, however the glycerol and melizitose utilization was positive for 50% or less of the HR isolates. Catalase activity (27.78% at INEL, 74.07% at HR) and tryptophan metabolism (11.12% at INEL, 40.74% at HR) were significantly different between the two study sites. MPN and viable counts indicate that sediments near the water table yield the greatest numbers of anaerobes. Deeper sediments also appear to be more selective with the greatest number of viable counts on low-nutrient mediums. Likewise, only strictly obligate anaerobes were found in the deepest sediment samples. Selective media indicated the presence of methanogens, acetogens, and sulfate reducers at only the HR site.
- Research Organization:
- Memphis State Univ., TN (United States). Dept. of Biology
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG05-90ER60991
- OSTI ID:
- 10108972
- Report Number(s):
- DOE/ER/60991--T3; ON: DE94004417; BR: KP0203000
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANAEROBIC CONDITIONS
BACTERIA
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IDAHO NATIONAL ENGINEERING LABORATORY
MICROBIOLOGY
MORPHOLOGY
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11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS
54 ENVIRONMENTAL SCIENCES
540250
550700
59 BASIC BIOLOGICAL SCIENCES
ANAEROBIC CONDITIONS
BACTERIA
ECOLOGY
EXPERIMENTAL DATA
HANFORD RESERVATION
HEALTH AND SAFETY
IDAHO NATIONAL ENGINEERING LABORATORY
MICROBIOLOGY
MORPHOLOGY
PHYSIOLOGY
POPULATION DYNAMICS
PROGRESS REPORT
REMEDIAL ACTION
SAMPLING
SEDIMENTS
SITE CHARACTERIZATION
SITE RESOURCE AND USE STUDIES