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U.S. Department of Energy
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Physiologically anaerobic microorganisms of the deep subsurface. Progress report, June 1, 1991--May 31, 1992

Technical Report ·
DOI:https://doi.org/10.2172/10157408· OSTI ID:10157408
A variety of different media were used to isolate facultatively (FAB) and obligately anaerobic bacteria (OAB). These bacteria were isolated from core subsamples obtained from boreholes at the Idaho National Engineering Lab. (INEL) or at the Hanford Lab. (Yakima). Core material was sampled at various depths to 600 feet below the surface. All core samples with culturable bacteria contained at least FAB making thisthe most common physiological type of anaerobic bacteria present in the deep subsurface at these two sites. INEL core samples are characterized by isolates of both FAB and OAB. No isolates of acetogenic, methanogenic, or sulfate reducing bacteria were obtained. Yakima core samples are characterized by a marked predominance of FAB in comparison to OAB. In addition, isolates of acetogenic, methanogenic, and sulfate reducing bacteria were obtained. The Yakima site has the potential for complete anaerobic mineralization of organic compounds whereas this potential appears to be lacking at INEL.
Research Organization:
Memphis State Univ., TN (United States). Dept. of Biology
Sponsoring Organization:
USDOE, Washington, DC (United States)
DOE Contract Number:
FG05-90ER60991
OSTI ID:
10157408
Report Number(s):
DOE/ER/60991--T2; ON: DE93015562
Country of Publication:
United States
Language:
English