Chemoselective ligation

Saxon, Eliana; Bertozzi, Carolyn R

Chemoselective ligation

Patent · Tue Apr 12 04:00:00 EDT 2011

OSTI ID:1016735

Saxon, Eliana ^[1]; Bertozzi, Carolyn R ^[2]

Albany, CA
Berkeley, CA

The present invention features a chemoselective ligation reaction that can be carried out under physiological conditions. In general, the invention involves condensation of a specifically engineered phosphine, which can provide for formation of an amide bond between the two reactive partners resulting in a final product comprising a phosphine moiety, or which can be engineered to comprise a cleavable linker so that a substituent of the phosphine is transferred to the azide, releasing an oxidized phosphine byproduct and producing a native amide bond in the final product. The selectivity of the reaction and its compatibility with aqueous environments provides for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).

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Research Organization:: The Regents of the University of California (Oakland, CA)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC03-76SF00098

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 7,923,582

Application Number:: US Patent Application 11/039,478

OSTI ID:: 1016735

Country of Publication:: United States

Language:: English

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