Chemoselective ligation

Saxon, Eliana; Bertozzi, Carolyn

Chemoselective ligation

Patent · Tue May 27 04:00:00 EDT 2003

OSTI ID:921799

Saxon, Eliana ^[1]; Bertozzi, Carolyn ^[2]

Albany, CA
Berkeley, CA

The present invention features a chemoselective ligation reaction that can be carried out under physiological conditions. In general, the invention involves condensation of a specifically engineered phosphine, which can provide for formation of an amide bond between the two reactive partners resulting in a final product comprising a phosphine moiety, or which can be engineered to comprise a cleavable linker so that a substituent of the phosphine is transferred to the azide, releasing an oxidized phosphine byproduct and producing a native amide bond in the final product. The selectivity of the reaction and its compatibility with aqueous environments provides for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA

Sponsoring Organization:: United States Department of Energy

DOE Contract Number:: AC03-76SF00098

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 6,570,040

Application Number:: 09/810,864

OSTI ID:: 921799

Country of Publication:: United States

Language:: English

References (21)

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