Time sequence of events leading to chromosomal aberration formation

Conference · Sat May 01 04:00:00 EDT 1993

OSTI ID:10154350

Moore, R C ^[1]; Bender, M A ^[2]

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1. Peter MacCallum Cancer Inst., Melbourne, VIC (Australia)
2. Brookhaven National Lab., Upton, NY (United States)

Investigations have been carried out on the influence of the repair polymerases on the yield of different types of chromosomal aberrations. The studies were mainly concerned with the effect of inhibiting the polymerases on the yield of aberrations. The polymerases fill in single-strand regions, and the fact that their inhibition affects the yield of aberrations suggests that single-strand lesions are influential in aberration formation. The results indicate that there are two actions of polymerases in clastogenesis. One is in their involvement in a G{sub 2} repair system, in which either of the two chromatids is concerned, and which does not yield aberrations unless the inhibition is still operating when the cells enter mitosis. The second is such that when repair is inhibited, further damage accrues. The second action is affected by inhibiting polymerase repair, but also operates even when the repair enzymes are active. The production of chromosomal exchanges involves a series of reactions, some of which are reversible. The time span over which the reactions occur is much longer than has been envisaged previously.

• Research Organization: Brookhaven National Lab., Upton, NY (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 10154350
• Report Number(s): BNL--48723; CONF-930295--2; ON: DE93013339
• Country of Publication: United States
• Language: English

Related Subjects

550200
560120
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMISTRY
CELL CYCLE
CHO CELLS
CHROMOSOMAL ABERRATIONS
DNA REPAIR
ENDONUCLEASES
ENZYME INHIBITORS
ETIOLOGY
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
RADIATION INDUCED MUTANTS
SISTER CHROMATID EXCHANGES
STRAND BREAKS
X RADIATION