Biomolecular hybrid material and process for preparing same and uses for same

Kim, Jungbae

Title: Biomolecular hybrid material and process for preparing same and uses for same

Patent · Tue Nov 23 00:00:00 EST 2010

OSTI ID:1014754

Kim, Jungbae ^[1]

Richland, WA

Disclosed is a composition and method for fabricating novel hybrid materials comprised of, e.g., carbon nanotubes (CNTs) and crosslinked enzyme clusters (CECs). In one method, enzyme-CNT hybrids are prepared by precipitation of enzymes which are subsequently crosslinked, yielding crosslinked enzyme clusters (CECs) on the surface of the CNTs. The CEC-enzyme-CNT hybrids exhibit high activity per unit area or mass as well as improved enzyme stability and longevity over hybrid materials known in the art. The CECs in the disclosed materials permit multilayer biocatalytic coatings to be applied to surfaces providing hybrid materials suitable for use in, e.g., biocatalytic applications and devices as described herein.

View Patent

Cite

Export

Save

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

Assignee:: Battelle Memorial Institute (Richland, WA)

Patent Number(s):: 7,838,273

Application Number:: US Patent Application 12/053,373

OSTI ID:: 1014754

Country of Publication:: United States

Language:: English

References (30)

Functionalization of Carbon Nanotubes by Electrochemical Reduction of Aryl Diazonium Salts: A Bucky Paper Electrode Bahr, Jeffrey L.; Yang, Jiping; Kosynkin, Dmitry V. Journal of the American Chemical Society, Vol. 123, Issue 27 https://doi.org/10.1021/ja010462s	journal	July 2001
Stabilisation of oxygen-labile nitrilases via co-aggregation with poly(ethyleneimine) Mateo, Cesar; Fernandes, Bruno; van Rantwijk, Fred Journal of Molecular Catalysis B: Enzymatic, Vol. 38, Issue 3-6 https://doi.org/10.1016/j.molcatb.2005.12.007	journal	March 2006
Attaching Proteins to Carbon Nanotubes via Diimide-Activated Amidation Huang, Weijie; Taylor, Shelby; Fu, Kefu Nano Letters, Vol. 2, Issue 4 https://doi.org/10.1021/nl010095i	journal	April 2002
Challenges in biocatalysis for enzyme-based biofuel cells Kim, Jungbae; Jia, Hongfei; Wang, Ping Biotechnology Advances, Vol. 24, Issue 3, p. 296-308 https://doi.org/10.1016/j.biotechadv.2005.11.006	journal	May 2006
Sidewall Carboxylic Acid Functionalization of Single-Walled Carbon Nanotubes Peng, Haiqing; Alemany, Lawrence B.; Margrave, John L. Journal of the American Chemical Society, Vol. 125, Issue 49 https://doi.org/10.1021/ja037746s	journal	December 2003
Heterogeneous Electron-Transfer Kinetics for Flavin Adenine Dinucleotide and Ferrocene through Alkanethiol Mixed Monolayers on Gold Electrodes The Journal of Physical Chemistry B, Vol. 108, Issue 24, p. 8460-8466 https://doi.org/10.1021/jp037494h	journal	May 2004
Miniature biofuel cells Heller, Adam Physical Chemistry Chemical Physics, Vol. 6, Issue 2 https://doi.org/10.1039/b313149a	journal	January 2004
Noncovalent Sidewall Functionalization of Single-Walled Carbon Nanotubes for Protein Immobilization Chen, Robert J.; Zhang, Yuegang; Wang, Dunwei Journal of the American Chemical Society, Vol. 123, Issue 16 https://doi.org/10.1021/ja010172b	journal	April 2001
Nanotube Molecular Transporters: Internalization of Carbon Nanotube−Protein Conjugates into Mammalian Cells Shi Kam, Nadine Wong; Jessop, Theodore C.; Wender, Paul A. Journal of the American Chemical Society, Vol. 126, Issue 22 https://doi.org/10.1021/ja0486059	journal	June 2004
Carbon Nanotube Purification: Preparation and Characterization of Carbon Nanotube Paste Electrodes Valentini, Federica; Amine, Aziz; Orlanducci, Silvia Analytical Chemistry, Vol. 75, Issue 20 https://doi.org/10.1021/ac0300237	journal	October 2003
Synthesis of enantiomerically pure (S)-mandelic acid using an oxynitrilase–nitrilase bienzymatic cascade: a nitrilase surprisingly shows nitrile hydratase activity Mateo, Cesar; Chmura, Andrzej; Rustler, Sven Tetrahedron: Asymmetry, Vol. 17, Issue 3 https://doi.org/10.1016/j.tetasy.2006.01.020	journal	February 2006
Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs) Schoevaart, R.; Wolbers, M. W.; Golubovic, M. Biotechnology and Bioengineering, Vol. 87, Issue 6 https://doi.org/10.1002/bit.20184	journal	January 2004
Cross-Linked Aggregates of ( R )-Oxynitrilase: A Stable, Recyclable Biocatalyst for Enantioselective Hydrocyanation van Langen, Luuk M.; Selassa, Rhoderick P.; van Rantwijk, Fred Organic Letters, Vol. 7, Issue 2 https://doi.org/10.1021/ol047647z	journal	January 2005
Enzymatic Biofuel Cells for Implantable and Microscale Devices Calabrese Barton, Scott; Gallaway, Josh; Atanassov, Plamen Chemical Reviews, Vol. 104, Issue 10 https://doi.org/10.1021/cr020719k	journal	October 2004
Preparation of biocatalytic nanofibres with high activity and stability via enzyme aggregate coating on polymer nanofibres Kim, Byoung Chan; Nair, Sujith; Kim, Jungbae Nanotechnology, Vol. 16, Issue 7 https://doi.org/10.1088/0957-4484/16/7/011	journal	April 2005
Preparation of a Magnetically Switchable Bio-electrocatalytic System Employing Cross-linked Enzyme Aggregates in Magnetic Mesocellular Carbon Foam Lee, Jinwoo; Lee, Dohoon; Oh, Eunkeu Angewandte Chemie International Edition, Vol. 44, Issue 45 https://doi.org/10.1002/anie.200502995	journal	November 2005
Protein engineering in bioelectrocatalysis Wong, Tuck Seng; Schwaneberg, Ulrich Current Opinion in Biotechnology, Vol. 14, Issue 6 https://doi.org/10.1016/j.copbio.2003.09.008	journal	December 2003
Biofuel cells and their development Bullen, R. A.; Arnot, T. C.; Lakeman, J. B. Biosensors and Bioelectronics, Vol. 21, Issue 11 https://doi.org/10.1016/j.bios.2006.01.030	journal	May 2006
Penicillin Acylase Catalysed Synthesis of Ampicillin in Hydrophilic Organic Solvents van Langen, Luuk M.; Oosthoek, Natasja H. P.; van Rantwijk, Fred Advanced Synthesis & Catalysis, Vol. 345, Issue 67 https://doi.org/10.1002/adsc.200303023	journal	June 2003
Miniature Biofuel Cells with Improved Stability Under Continuous Operation Fischback, Michael B.; Youn, Jong Kyu; Zhao, Xueyan Electroanalysis, Vol. 18, Issue 19-20 https://doi.org/10.1002/elan.200603626	journal	October 2006
Preparation of a highly stable, very active and high-yield multilayered assembly of glucose oxidase using carbohydrate-specific polyclonal antibodies Jan, Ulfat; Husain, Qayyum Biotechnology and Applied Biochemistry, Vol. 39, Issue 2 https://doi.org/10.1042/BA20030092	journal	April 2004
Carbon-Nanotube Based Electrochemical Biosensors: A Review Wang, Joseph Electroanalysis, Vol. 17, Issue 1 https://doi.org/10.1002/elan.200403113	journal	January 2005
Cross-linked enzyme aggregates (CLEAs): A novel and versatile method for enzyme immobilization (a review) Sheldon, R. A.; Schoevaart, R.; Van Langen, L. M. Biocatalysis and Biotransformation, Vol. 23, Issue 3-4 https://doi.org/10.1080/10242420500183378	journal	January 2005
A new, mild cross-linking methodology to prepare cross-linked enzyme aggregates Mateo, Cesar; Palomo, Jos� M.; van Langen, Luuk M. Biotechnology and Bioengineering, Vol. 86, Issue 3 https://doi.org/10.1002/bit.20033	journal	January 2004
Enzyme−Polymer−Single Walled Carbon Nanotube Composites as Biocatalytic Films Rege, Kaushal; Raravikar, Nachiket R.; Kim, Dae-Yun Nano Letters, Vol. 3, Issue 6 https://doi.org/10.1021/nl034131k	journal	June 2003
Bioelectric power generation Palmore, G. Tayhas R. Trends in Biotechnology, Vol. 22, Issue 3 https://doi.org/10.1016/j.tibtech.2004.01.004	journal	March 2004
Simple Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica Materials Hosting Crosslinked Enzyme Aggregates Lee, Jinwoo; Kim, Jungbae; Kim, Jaeyun Small, Vol. 1, Issue 7 https://doi.org/10.1002/smll.200500035	journal	July 2005
Simple Fabrication of a Highly Sensitive and Fast Glucose Biosensor Using Enzymes Immobilized in Mesocellular Carbon Foam Lee, D.; Lee, J.; Kim, J. Advanced Materials, Vol. 17, Issue 23 https://doi.org/10.1002/adma.200500793	journal	December 2005
Amperometric Biosensors Based on Redox Polymer−Carbon Nanotube−Enzyme Composites Joshi, Pratixa P.; Merchant, Stephen A.; Wang, Youdan Analytical Chemistry, Vol. 77, Issue 10 https://doi.org/10.1021/ac0484169	journal	May 2005
Protein immobilization on carbon nanotubes via a two-step process of diimide-activated amidation Jiang, Kuiyang; Schadler, Linda S.; Siegel, Richard W. Journal of Materials Chemistry, Vol. 14, Issue 1 https://doi.org/10.1039/B310359E	journal	January 2004

Similar Records

Process for preparing multilayer enzyme coating on a fiber

Patent · Tue Nov 03 00:00:00 EST 2009 · OSTI ID:1014754

Kim, Jungbae; Kwak, Ja Hun; Grate, Jay W

Miniature Biofuel Cells with Improved Stability under Continuous Operation

Journal Article · Sun Oct 01 00:00:00 EDT 2006 · Electroanalysis, 18(19-20):2016-2022 · OSTI ID:1014754

Fishback, Michael; Youn, Jongkyu; Zhao, Xueyan; +5 more

Novel method for carbon nanofilament growth on carbon fibers

Journal Article · Thu Jan 01 00:00:00 EST 2009 · Advanced Materials · OSTI ID:1014754

Phillips, Johathan; Luhrs, Claudia; Terani, Mehran; +3 more

Title: Biomolecular hybrid material and process for preparing same and uses for same

Citation Formats

References (30)

Similar Records

Related Subjects