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Title: Process for preparing multilayer enzyme coating on a fiber

Abstract

A process for preparing high stability, high activity biocatalytic materials is disclosed and processes for using the same. The process involves coating of a material or fiber with enzymes and enzyme aggregate providing a material or fiber with high biocatalytic activity and stability useful in heterogeneous environments. In one illustrative approach, enzyme "seeds" are covalently attached to polymer nanofibers followed by treatment with a reagent that crosslinks additional enzyme molecules to the seed enzymes forming enzyme aggregates thereby improving biocatalytic activity due to increased enzyme loading and enzyme stability. This approach creates a useful new biocatalytic immobilized enzyme system with potential applications in bioconversion, bioremediation, biosensors, and biofuel cells.

Inventors:
 [1];  [1];  [2]
  1. Richland, WA
  2. West Richland, WA
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
982369
Patent Number(s):
7,611,835
Application Number:
11/241,506
Assignee:
Battelle Memorial Institute (Richland, WA)
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Kim, Jungbae, Kwak, Ja Hun, and Grate, Jay W. Process for preparing multilayer enzyme coating on a fiber. United States: N. p., 2009. Web.
Kim, Jungbae, Kwak, Ja Hun, & Grate, Jay W. Process for preparing multilayer enzyme coating on a fiber. United States.
Kim, Jungbae, Kwak, Ja Hun, and Grate, Jay W. Tue . "Process for preparing multilayer enzyme coating on a fiber". United States. https://www.osti.gov/servlets/purl/982369.
@article{osti_982369,
title = {Process for preparing multilayer enzyme coating on a fiber},
author = {Kim, Jungbae and Kwak, Ja Hun and Grate, Jay W},
abstractNote = {A process for preparing high stability, high activity biocatalytic materials is disclosed and processes for using the same. The process involves coating of a material or fiber with enzymes and enzyme aggregate providing a material or fiber with high biocatalytic activity and stability useful in heterogeneous environments. In one illustrative approach, enzyme "seeds" are covalently attached to polymer nanofibers followed by treatment with a reagent that crosslinks additional enzyme molecules to the seed enzymes forming enzyme aggregates thereby improving biocatalytic activity due to increased enzyme loading and enzyme stability. This approach creates a useful new biocatalytic immobilized enzyme system with potential applications in bioconversion, bioremediation, biosensors, and biofuel cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {11}
}

Patent:

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