Process for preparing multilayer enzyme coating on a fiber

Kim, Jungbae; Kwak, Ja Hun; Grate, Jay W

Process for preparing multilayer enzyme coating on a fiber

Patent · Tue Nov 03 04:00:00 EST 2009

OSTI ID:982369

Kim, Jungbae ^[1]; Kwak, Ja Hun ^[1]; Grate, Jay W ^[2]

Richland, WA
West Richland, WA

A process for preparing high stability, high activity biocatalytic materials is disclosed and processes for using the same. The process involves coating of a material or fiber with enzymes and enzyme aggregate providing a material or fiber with high biocatalytic activity and stability useful in heterogeneous environments. In one illustrative approach, enzyme "seeds" are covalently attached to polymer nanofibers followed by treatment with a reagent that crosslinks additional enzyme molecules to the seed enzymes forming enzyme aggregates thereby improving biocatalytic activity due to increased enzyme loading and enzyme stability. This approach creates a useful new biocatalytic immobilized enzyme system with potential applications in bioconversion, bioremediation, biosensors, and biofuel cells.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

Assignee:: Battelle Memorial Institute (Richland, WA)

Patent Number(s):: 7,611,835

Application Number:: 11/241,506

OSTI ID:: 982369

Country of Publication:: United States

Language:: English

References (21)

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