skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Process for preparing multilayer enzyme coating on a fiber

Abstract

A process for preparing high stability, high activity biocatalytic materials is disclosed and processes for using the same. The process involves coating of a material or fiber with enzymes and enzyme aggregate providing a material or fiber with high biocatalytic activity and stability useful in heterogeneous environments. In one illustrative approach, enzyme "seeds" are covalently attached to polymer nanofibers followed by treatment with a reagent that crosslinks additional enzyme molecules to the seed enzymes forming enzyme aggregates thereby improving biocatalytic activity due to increased enzyme loading and enzyme stability. This approach creates a useful new biocatalytic immobilized enzyme system with potential applications in bioconversion, bioremediation, biosensors, and biofuel cells.

Inventors:
 [1];  [1];  [2]
  1. Richland, WA
  2. West Richland, WA
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
982369
Patent Number(s):
7,611,835
Application Number:
11/241,506
Assignee:
Battelle Memorial Institute (Richland, WA)
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Kim, Jungbae, Kwak, Ja Hun, and Grate, Jay W. Process for preparing multilayer enzyme coating on a fiber. United States: N. p., 2009. Web.
Kim, Jungbae, Kwak, Ja Hun, & Grate, Jay W. Process for preparing multilayer enzyme coating on a fiber. United States.
Kim, Jungbae, Kwak, Ja Hun, and Grate, Jay W. 2009. "Process for preparing multilayer enzyme coating on a fiber". United States. https://www.osti.gov/servlets/purl/982369.
@article{osti_982369,
title = {Process for preparing multilayer enzyme coating on a fiber},
author = {Kim, Jungbae and Kwak, Ja Hun and Grate, Jay W},
abstractNote = {A process for preparing high stability, high activity biocatalytic materials is disclosed and processes for using the same. The process involves coating of a material or fiber with enzymes and enzyme aggregate providing a material or fiber with high biocatalytic activity and stability useful in heterogeneous environments. In one illustrative approach, enzyme "seeds" are covalently attached to polymer nanofibers followed by treatment with a reagent that crosslinks additional enzyme molecules to the seed enzymes forming enzyme aggregates thereby improving biocatalytic activity due to increased enzyme loading and enzyme stability. This approach creates a useful new biocatalytic immobilized enzyme system with potential applications in bioconversion, bioremediation, biosensors, and biofuel cells.},
doi = {},
url = {https://www.osti.gov/biblio/982369}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {11}
}

Works referenced in this record:

Cross-linked aggregates of penicillin acylase: robust catalysts for the synthesis of β-lactam antibiotics
journal, January 2001


Enzyme-Carrying Polymeric Nanofibers Prepared via Electrospinning for Use as Unique Biocatalysts
journal, October 2002


Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning
journal, January 2005


Stabilisation of oxygen-labile nitrilases via co-aggregation with poly(ethyleneimine)
journal, March 2006


Penicillin Acylase Catalysed Synthesis of Ampicillin in Hydrophilic Organic Solvents
journal, June 2003


Immobilized Hydroxynitrile Lyases for Enantioselective Synthesis of Cyanohydrins: Sol-Gels and Cross-Linked Enzyme Aggregates
journal, August 2006


Miniature Biofuel Cells with Improved Stability Under Continuous Operation
journal, October 2006


Cross-linked enzyme aggregates (CLEAs): A novel and versatile method for enzyme immobilization (a review)
journal, January 2005


Continuous kinetic resolution catalysed by cross-linked enzyme aggregates, ‘CLEAs’, in supercritical CO 2
journal, January 2006


Electrospinning of Polymeric and Ceramic Nanofibers as Uniaxially Aligned Arrays
journal, August 2003


A new, mild cross-linking methodology to prepare cross-linked enzyme aggregates
journal, January 2004


Crude aminoacylase fromaspergillus sp. is a mixture of hydrolases
journal, October 2003


Immobilised enzymes: carrier-bound or carrier-free?
journal, August 2003


Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs)
journal, January 2004


Cross-Linked Aggregates of ( R )-Oxynitrilase:  A Stable, Recyclable Biocatalyst for Enantioselective Hydrocyanation
journal, January 2005


Cross-Linked Enzyme Aggregates:  A Simple and Effective Method for the Immobilization of Penicillin Acylase
journal, May 2000


Cross-Linked Aggregates of the Hydroxynitrile Lyase fromManihot esculenta: Highly Active and Robust Biocatalysts
journal, August 2006