Convergent synthesis of proteins by kinetically controlled ligation

Kent, Stephen; Pentelute, Brad; Bang, Duhee; Johnson, Erik; Durek, Thomas

Convergent synthesis of proteins by kinetically controlled ligation

Patent · 08 March 2010

OSTI ID:1014499

Kent, Stephen ^[1]; Pentelute, Brad ^[1]; Bang, Duhee ^[2]; Johnson, Erik ^[1]; Durek, Thomas ^[1]

Chicago, IL
Boston, MA

The present invention concerns methods and compositions for synthesizing a polypeptide using kinetically controlled reactions involving fragments of the polypeptide for a fully convergent process. In more specific embodiments, a ligation involves reacting a first peptide having a protected cysteyl group at its N-terminal and a phenylthioester at its C-terminal with a second peptide having a cysteine residue at its N-termini and a thioester at its C-termini to form a ligation product. Subsequent reactions may involve deprotecting the cysteyl group of the resulting ligation product and/or converting the thioester into a thiophenylester.

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Research Organization:: The University of Chicago (Chicago, IL)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-04ER63786

Assignee:: The University of Chicago (Chicago, IL)

Patent Number(s):: 7,674,881

Application Number:: U S Patent Application 11/545,923

OSTI ID:: 1014499

Country of Publication:: United States

Language:: English

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