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Title: Convergent synthesis of proteins by kinetically controlled ligation

Abstract

The present invention concerns methods and compositions for synthesizing a polypeptide using kinetically controlled reactions involving fragments of the polypeptide for a fully convergent process. In more specific embodiments, a ligation involves reacting a first peptide having a protected cysteyl group at its N-terminal and a phenylthioester at its C-terminal with a second peptide having a cysteine residue at its N-termini and a thioester at its C-termini to form a ligation product. Subsequent reactions may involve deprotecting the cysteyl group of the resulting ligation product and/or converting the thioester into a thiophenylester.

Inventors:
 [1];  [1];  [2];  [1];  [1]
  1. (Chicago, IL)
  2. (Boston, MA)
Publication Date:
Research Org.:
The University of Chicago (Chicago, IL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1014499
Patent Number(s):
7,674,881
Application Number:
U S Patent Application 11/545,923
Assignee:
The University of Chicago (Chicago, IL) CHO
DOE Contract Number:  
FG02-04ER63786
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Kent, Stephen, Pentelute, Brad, Bang, Duhee, Johnson, Erik, and Durek, Thomas. Convergent synthesis of proteins by kinetically controlled ligation. United States: N. p., 2010. Web.
Kent, Stephen, Pentelute, Brad, Bang, Duhee, Johnson, Erik, & Durek, Thomas. Convergent synthesis of proteins by kinetically controlled ligation. United States.
Kent, Stephen, Pentelute, Brad, Bang, Duhee, Johnson, Erik, and Durek, Thomas. Tue . "Convergent synthesis of proteins by kinetically controlled ligation". United States. https://www.osti.gov/servlets/purl/1014499.
@article{osti_1014499,
title = {Convergent synthesis of proteins by kinetically controlled ligation},
author = {Kent, Stephen and Pentelute, Brad and Bang, Duhee and Johnson, Erik and Durek, Thomas},
abstractNote = {The present invention concerns methods and compositions for synthesizing a polypeptide using kinetically controlled reactions involving fragments of the polypeptide for a fully convergent process. In more specific embodiments, a ligation involves reacting a first peptide having a protected cysteyl group at its N-terminal and a phenylthioester at its C-terminal with a second peptide having a cysteine residue at its N-termini and a thioester at its C-termini to form a ligation product. Subsequent reactions may involve deprotecting the cysteyl group of the resulting ligation product and/or converting the thioester into a thiophenylester.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 09 00:00:00 EST 2010},
month = {Tue Mar 09 00:00:00 EST 2010}
}

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