Rapid classification of biological components

Thompson, Vicki S; Barrett, Karen B; Key, Diane E

Rapid classification of biological components

Patent · 23 March 2010

OSTI ID:1014468

Thompson, Vicki S ^[1]; Barrett, Karen B ^[2]; Key, Diane E ^[1]

Idaho Falls, ID
Meridian, ID

A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

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Research Organization:: Idaho National Laboratory (INL), Idaho Falls, ID (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC07-94ID13223; AC07-99ID13727; AC07-05ID14517

Assignee:: Battelle Energy Alliance, LLC (Idaho Falls, ID)

Patent Number(s):: 7,682,797

Application Number:: U S Patent Application 11/101,216

OSTI ID:: 1014468

Country of Publication:: United States

Language:: English

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