Leptin Increases Striatal Dopamine D2 Receptor Binding in Leptin-Deficient Obese (ob/ob) Mice

Journal Article · Tue Jun 01 04:00:00 EDT 2010 · PLoS One

DOI:https://doi.org/10.1002/syn.20755· OSTI ID:1014309

Pfaffly, J; Michaelides, M; Wang, G-J; Pessin, J E; Volkow, N D; Thanos, P K

Peripheral and central leptin administration have been shown to mediate central dopamine (DA) signaling. Leptin-receptor deficient rodents show decreased DA D2 receptor (D2R) binding in striatum and unique DA profiles compared to controls. Leptin-deficient mice show increased DA activity in reward-related brain regions. The objective of this study was to examine whether basal D2R-binding differences contribute to the phenotypic behaviors of leptin-deficient ob/ob mice, and whether D2R binding is altered in response to peripheral leptin treatment in these mice. Leptin decreased body weight, food intake, and plasma insulin concentration in ob/ob mice but not in wild-type mice. Basal striatal D2R binding (measured with autoradiography [{sup 3}H] spiperone) did not differ between ob/ob and wild-type mice but the response to leptin did. In wild-type mice, leptin decreased striatal D2R binding, whereas, in ob/ob mice, leptin increased D2R binding. Our findings provide further evidence that leptin modulates D2R expression in striatum and that these effects are genotype/phenotype dependent.

• Research Organization: BROOKHAVEN NATIONAL LABORATORY (BNL)
• Sponsoring Organization: NATIONAL INSTITUTE ON ALCOHOL ABUSE & ALCOHOLISM
• DOE Contract Number: AC02-98CH10886
• OSTI ID: 1014309
• Report Number(s): BNL--93781-2010-JA; KP1503010
• Journal Information: PLoS One, Journal Name: PLoS One Journal Issue: 7 Vol. 64
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS
AUTORADIOGRAPHY
BRAIN
Cocaine
DOPAMINE
Dopamine
FOOD
INSULIN
LEPTIN
Leptin
MICE
PLASMA
RODENTS
SPIPERONE