Autoradiography of dopamine receptors and dopamine uptake sites in the spontaneously hypertensive rat

Journal Article · Wed Oct 31 23:00:00 EST 1990 · Brain Research Bulletin; (USA)

DOI:https://doi.org/10.1016/0361-9230(90)90046-3· OSTI ID:5857635

Kujirai, K; Przedborski, S; Kostic, V; Jackson-Lewis, V; Fahn, S; Cadet, J L ^[1]

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1. Columbia Univ., New York, NY (USA)

We examined the status of dopamine (DA) D1 and D2 receptors by using (3H)SCH 23390 and (3H)spiperone binding, respectively, and DA uptake sites by using (3H)mazindol binding in spontaneously hypertensive rats (SHR) and Sprague-Dawley (SD) rats. SHR showed significantly higher (3H)SCH 23390 and (3H)spiperone binding in the caudate-putamen (CPu), the nucleus accumbens (NAc) and the olfactory tubercle (OT) in comparison to the SD rats. There were no significant differences in (3H)mazindol-labeled DA uptake sites between the two strains. Unilateral 6-hydroxydopamine (6-OHDA) injection into the striatum resulted in more than 90% depletion of DA uptake sites in the CPu in both strains. 6-OHDA-induced DA depletion was associated with significant increases in striatal (3H)spiperone binding which were of similar magnitude in the SD rats (+64.1%) and SHR (+51.3%). There were only small decreases (-5.4%) in D1 receptor binding in the dorsolateral aspect of the CPu in the SHR, whereas there were no changes in striatal D1 receptors in the SD rats. These results indicate that, although the SHR have higher concentrations of both D1 and D2 receptors in the basal ganglia, these receptors are regulated in a fashion similar to DA receptors in SD rats after 6-OHDA-induced striatal DA depletion.

• OSTI ID: 5857635
• Journal Information: Brain Research Bulletin; (USA), Journal Name: Brain Research Bulletin; (USA) Vol. 25:5; ISSN BRBUD; ISSN 0361-9230
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
DISEASES
DOPAMINE
DRUGS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
HYPERTENSION
KINETICS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHENOLS
POLYPHENOLS
PROTEINS
RADIOASSAY
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SPIPERONE
SYMPATHOMIMETICS
SYMPTOMS
TRITIUM COMPOUNDS
UPTAKE
VASCULAR DISEASES
VERTEBRATES