A subambient pressure ionization with nanoelectrospray (SPIN) source and interface for improved sensitivity in mass spectrometry
Journal Article
·
· Analytical Chemistry, 80(5):1800-1805
Subambient Pressure Ionization with Nanoelectrospray (SPIN), an electrospray ionization source that operates at 30 Torr inside the first vacuum chamber of a mass spectrometer, has been demonstrated for reversed-phase liquid chromatography-mass spectrometry analysis of a protein tryptic digest solution. A 5–12-fold improvement in sensitivity relative to a standard atmospheric pressure ESI source was observed for a variety of detected peptides. The low liquid chromatographic flow rate (300 nL/min) allowed stable electrospray to be established before the onset of electrical discharge, and the higher operating pressure of the SPIN source relative to previous low-pressure ESI source designs prevented the solvents from freezing. The range of accessible flow rates for the SPIN source was also extended to 2.5 μL/min by using an array of electrospray emitters that divided the flow to 6 discrete electrosprays.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1013315
- Report Number(s):
- PNNL-SA-60054; 400412000
- Journal Information:
- Analytical Chemistry, 80(5):1800-1805, Journal Name: Analytical Chemistry, 80(5):1800-1805 Journal Issue: 5 Vol. 80
- Country of Publication:
- United States
- Language:
- English
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