A subambient pressure ionization with nanoelectrospray (SPIN) source and interface for improved sensitivity in mass spectrometry
Subambient Pressure Ionization with Nanoelectrospray (SPIN), an electrospray ionization source that operates at 30 Torr inside the first vacuum chamber of a mass spectrometer, has been demonstrated for reversed-phase liquid chromatography-mass spectrometry analysis of a protein tryptic digest solution. A 5–12-fold improvement in sensitivity relative to a standard atmospheric pressure ESI source was observed for a variety of detected peptides. The low liquid chromatographic flow rate (300 nL/min) allowed stable electrospray to be established before the onset of electrical discharge, and the higher operating pressure of the SPIN source relative to previous low-pressure ESI source designs prevented the solvents from freezing. The range of accessible flow rates for the SPIN source was also extended to 2.5 μL/min by using an array of electrospray emitters that divided the flow to 6 discrete electrosprays.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1013315
- Report Number(s):
- PNNL-SA-60054; 400412000; TRN: US201110%%500
- Journal Information:
- Analytical Chemistry, 80(5):1800-1805, Vol. 80, Issue 5
- Country of Publication:
- United States
- Language:
- English
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Subambient pressure ionization with nanoelectrospray source and interface for improved sensitivity in mass spectrometry