RNA binding protein and binding site useful for expression of recombinant molecules
Patent
·
OSTI ID:873426
- Cardiff, CA
The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.
- Research Organization:
- Scripps Research Institute (La Jolla, CA)
- DOE Contract Number:
- FG03-93ER20116
- Assignee:
- Scripps Research Institute (La Jolla, CA)
- Patent Number(s):
- US 6156517
- OSTI ID:
- 873426
- Country of Publication:
- United States
- Language:
- English
Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins.
|
journal | December 1991 |
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Related Subjects
rna
binding
protein
site
useful
expression
recombinant
molecules
relates
eukaryotic
prokaryotic
cells
preferably
plant
intact
plants
particular
rb47
upstream
translation
initiation
regulation
mediated
poly
family
effected
rb60
disulfide
isomerase
capable
functioning
nuclear
cytoplasm
chloroplast
desired
molecule
enhanced
approximately
100
fold
obtained
activation
binding site
binding protein
plant cell
plant cells
recombinant molecule
site useful
/435/999/
binding
protein
site
useful
expression
recombinant
molecules
relates
eukaryotic
prokaryotic
cells
preferably
plant
intact
plants
particular
rb47
upstream
translation
initiation
regulation
mediated
poly
family
effected
rb60
disulfide
isomerase
capable
functioning
nuclear
cytoplasm
chloroplast
desired
molecule
enhanced
approximately
100
fold
obtained
activation
binding site
binding protein
plant cell
plant cells
recombinant molecule
site useful
/435/999/