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Title: Expression of eukaryotic polypeptides in chloroplasts

Patent ·
OSTI ID:1084329

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

Research Organization:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-93ER20116
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
RE44,266
Application Number:
11/197,730
OSTI ID:
1084329
Country of Publication:
United States
Language:
English

References (20)

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