The role of proteolytic enzymes in degradation of plant tissues: Summary report
The proteolytic enzymes produced by Erwinia carotovora subsp. carotovora (Ecc-strain SR 394) grown on various media were examined by isoelectrofocusing in polyacrylamide gels over a pH range of 3-10. In addition to the main protease present in culture filtrates, low concentrations of several other proteases were present in extracts from potato tubers infected by Ecc. Proteases from all these sources were similar and had the following properties: pH optimum near 8.0, calcium dependent, insensitive to serine proteinase and SH-proteinase inhibitors, inhibited by EDTA, and highly thermostable. These enzymes degraded gelatin, soluble collagen and Hide Powder Azure, and showed weak activity on casein, but did not degrade insoluble collagen or elastin.
- Research Organization:
- Univ. of Wisconsin, Madison, WI (United States)
- DOE Contract Number:
- FG02-87ER13717
- OSTI ID:
- 6206504
- Report Number(s):
- DOE/ER/13717-1; ON: DE89013197
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Role of proteolytic enzymes in degradation of plant tissues
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CELLULOLYTIC ACTIVITY
PLANT TISSUES
BIODEGRADATION
BIOCHEMISTRY
CELLULASE
ENZYMATIC HYDROLYSIS
ION EXCHANGE CHROMATOGRAPHY
PH VALUE
PROGRESS REPORT
BODY
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
DECOMPOSITION
DOCUMENT TYPES
ENZYME ACTIVITY
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
SEPARATION PROCESSES
SOLVOLYSIS
TISSUES
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550200 - Biochemistry