Role of proteolytic enzymes in degradation of plant tissues
Strain SR 394 of Erwinia carotovora (Ecc) produced proteases constitutively in all media tested. Growth of Ecc and production of protease were enhanced significantly by the presence of poetic materials and/or plant call walls in the test media. After electrofocusing, one major and one minor protease bands, at PI 4.8 and PI 5.1, respectively, were detected. Only one band of 43 kDa was detected on SDS gels. Only one protease band was detected in SDS gels of infected plant extracts. This protease was purified to homogeneity. It in a highly thermostable metal protease; it degrades gelatin, soluble collagen and hide powderazure, shows weak activity on casein and azocasein, but does not degrade insoluble collagen or elastin.
- Research Organization:
- Wisconsin Univ., Madison, WI (United States). Dept. of Plant Pathology
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-87ER13717
- OSTI ID:
- 6445200
- Report Number(s):
- DOE/ER/13717-3; ON: DE93015740
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELL WALL
ENZYMATIC HYDROLYSIS
PEPTIDE HYDROLASES
ENZYME INDUCTION
GLYCOPROTEINS
HYDROXYPROLINE
PLANT DISEASES
AMINES
AMINO ACIDS
AZOLES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
GENE REGULATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROLYSIS
HYDROXY ACIDS
LYSIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRROLES
PYRROLIDINES
SOLVOLYSIS
090900* - Biomass Fuels- Processing- (1990-)
550200 - Biochemistry