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Title: Software for the high-throughput collection of SAXS data using an enhanced Blu-Ice / DCS control system

Journal Article · · Journal of Synchrotron Radiation
 [1];  [2];  [3];  [1];  [1];  [4]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division
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Biological small-angle X-ray scattering (SAXS) provides powerful complementary data for macromolecular crystallography (MX) by defining shape, conformation and assembly in solution. Although SAXS is in principle the highest throughput technique for structural biology, data collection is limited in practice by current data collection software. Here the adaption of beamline control software, historically developed for MX beamlines, for the efficient operation and high-throughput data collection at synchrotron SAXS beamlines is reported. The Blu-Ice GUI and Distributed Control System (DCS) developed in the Macromolecular Crystallography Group at the Stanford Synchrotron Radiation Laboratory has been optimized, extended and enhanced to suit the specific needs of the biological SAXS end station at the SIBYLS beamline at the Advanced Light Source. The customizations reported here provide a potential route for other SAXS beamlines in need of robust and efficient beamline control software. As a great deal of effort and optimization has gone into crystallographic software, the adaption and extension of crystallographic software may prove to be a general strategy to provide advanced SAXS software for the synchrotron community. In this way effort can be put into optimizing features for SAXS rather than reproducing those that have already been successfully implemented for the crystallographic community.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1625694
Journal Information:
Journal of Synchrotron Radiation, Vol. 17, Issue 6; ISSN 0909-0495
Publisher:
International Union of CrystallographyCopyright Statement
Country of Publication:
United States
Language:
English

Cited By (13)

Small angle X-ray scattering and cross-linking for data assisted protein structure prediction in CASP 12 with prospects for improved accuracy journal February 2018
Small angle X‐ray scattering‐assisted protein structure prediction in CASP13 and emergence of solution structure differences journal August 2019
SAXS studies of RNA: structures, dynamics, and interactions with partners: SAXS studies of RNA journal April 2016
High-Throughput SAXS for the Characterization of Biomolecules in Solution: A Practical Approach book October 2013
Exploring the repeat protein universe through computational protein design journal December 2015
Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5 journal June 2013
A new structural framework for integrating replication protein A into DNA processing machinery journal January 2013
Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy posted_content August 2019
Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5 text January 2013
Insights into FlaI Functions in Archaeal Motor Assembly and Motility from Structures, Conformations, and Genetics journal March 2013
Structural basis for recognition of 5′-phosphotyrosine adducts by Tdp2 journal October 2012
Structural and Biochemical Characterization of Human PR70 in Isolation and in Complex with the Scaffolding Subunit of Protein Phosphatase 2A journal July 2014
Wedging open a catalytic site journal November 2019

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Instruments & Instrumentation
Optics
Physics
SAXS
software
beamline
control system
Blu-Ice
DCS
SIBYLS
GUI