RNA binding protein and binding site useful for expression of recombinant molecules

Mayfield, Stephen P.

Title: RNA binding protein and binding site useful for expression of recombinant molecules

Patent · Tue Oct 17 00:00:00 EDT 2006

OSTI ID:1176706

Mayfield, Stephen P.

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

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Research Organization:: Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG03-93ER20116

Assignee:: Scripps Research Institute (La Jolla, CA)

Patent Number(s):: RE39350

Application Number:: 10/310,587; 6,156,517

OSTI ID:: 1176706

Resource Relation:: Patent File Date: 1998 Jan 16

Country of Publication:: United States

Language:: English

References (3)

Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins. Danon, A.; Mayfield, S. P. The EMBO Journal, Vol. 10, Issue 13 https://doi.org/10.1002/j.1460-2075.1991.tb04974.x	journal	December 1991
Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum D�ring, Klaus; Hippe, Sigrun; Kreuzaler, Fritz Plant Molecular Biology, Vol. 15, Issue 2 https://doi.org/10.1007/BF00036914	journal	August 1990
ADP-dependent phosphorylation regulates RNA-binding in vitro: implications in light-modulated translation. Danon, A.; Mayfield, S. P. The EMBO Journal, Vol. 13, Issue 9 https://doi.org/10.1002/j.1460-2075.1994.tb06500.x	journal	May 1994

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