The UzcRS two-component system in Caulobacter crescentus integrates regulatory input from diverse auxiliary regulators

Park, Dan M.; Wesley Overton, K.; Jiao, Yongqin

doi:10.1111/mmi.14180

Title: The UzcRS two-component system in Caulobacter crescentus integrates regulatory input from diverse auxiliary regulators

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Abstract

Summary The UzcRS two‐component system in Caulobacter crescentus mediates widespread transcriptional activation in response to the metals U, Zn and Cu. Unexpectedly, a screen for mutations that affected the activity of the UzcR‐regulated urcA promoter (P _urcA ) revealed four previously uncharacterized proteins whose inactivation led to metal‐independent induction of P _urcA . Using molecular genetics and functional genomics, we find that these auxiliary regulators control P _urcA expression by modulating the activity of UzcRS through distinct mechanisms. An ABC transporter with a periplasmic metallo‐aminopeptidase domain forms a sensory complex with UzcRS, antagonizing metal dependent stimulation by virtue of its ATPase and peptidase domains. Two MarR‐like transcription factors synergistically regulate UzcRS activity by repressing the expression of the membrane proteins UzcY and UzcZ, which stimulate UzcRS activity and enhance its sensitivity to a more environmentally relevant U/Zn/Cu concentration range. Additionally, the membrane protein UzcX, whose expression is positively regulated by UzcR, provides a mechanism of feedback inhibition within the UzcRS circuit. Collectively, these data suggest that UzcRS functions as the core‐signaling unit within a multicomponent signal transduction pathway that includes a diverse set of auxiliary regulators, providing further insight into the complexity of signalingmore » networks.« less

Authors:

Park, Dan M. ^[1]; Wesley Overton, K. ^[1]; Jiao, Yongqin ^[1]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Biosciences and Biotechnology Division. Physical and Life Sciences Directorate

Publication Date:: Sat Dec 08 00:00:00 EST 2018

Research Org.:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER); LLNL Laboratory Directed Research and Development (LDRD) Program; National Inst. of Health (NIH) (United States)

OSTI Identifier:: 1513159

Alternate Identifier(s):: OSTI ID: 1787157

Report Number(s):: LLNL-JRNL-743338
Journal ID: ISSN 0950-382X; 898425

Grant/Contract Number:: AC52-07NA27344; S10 OD018174

Resource Type:: Accepted Manuscript

Journal Name:: Molecular microbiology

Additional Journal Information:: Journal Volume: 111; Journal Issue: 3; Journal ID: ISSN 0950-382X

Publisher:: Wiley

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Park, Dan M., Wesley Overton, K., and Jiao, Yongqin. The UzcRS two-component system in Caulobacter crescentus integrates regulatory input from diverse auxiliary regulators.  United States: N. p., 2018. 
Web.  doi:10.1111/mmi.14180.

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                    Park, Dan M., Wesley Overton, K., & Jiao, Yongqin. The UzcRS two-component system in Caulobacter crescentus integrates regulatory input from diverse auxiliary regulators.  United States.  https://doi.org/10.1111/mmi.14180

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                    Park, Dan M., Wesley Overton, K., and Jiao, Yongqin. Sat .  
"The UzcRS two-component system in Caulobacter crescentus integrates regulatory input from diverse auxiliary regulators".  United States.  https://doi.org/10.1111/mmi.14180.  https://www.osti.gov/servlets/purl/1513159.

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@article{osti_1513159,

  title        = {The UzcRS two-component system in Caulobacter crescentus integrates regulatory input from diverse auxiliary regulators},

  author       = {Park, Dan M. and Wesley Overton, K. and Jiao, Yongqin},

  abstractNote = {Summary The UzcRS two‐component system in Caulobacter crescentus mediates widespread transcriptional activation in response to the metals U, Zn and Cu. Unexpectedly, a screen for mutations that affected the activity of the UzcR‐regulated urcA promoter (P urcA ) revealed four previously uncharacterized proteins whose inactivation led to metal‐independent induction of P urcA . Using molecular genetics and functional genomics, we find that these auxiliary regulators control P urcA expression by modulating the activity of UzcRS through distinct mechanisms. An ABC transporter with a periplasmic metallo‐aminopeptidase domain forms a sensory complex with UzcRS, antagonizing metal dependent stimulation by virtue of its ATPase and peptidase domains. Two MarR‐like transcription factors synergistically regulate UzcRS activity by repressing the expression of the membrane proteins UzcY and UzcZ, which stimulate UzcRS activity and enhance its sensitivity to a more environmentally relevant U/Zn/Cu concentration range. Additionally, the membrane protein UzcX, whose expression is positively regulated by UzcR, provides a mechanism of feedback inhibition within the UzcRS circuit. Collectively, these data suggest that UzcRS functions as the core‐signaling unit within a multicomponent signal transduction pathway that includes a diverse set of auxiliary regulators, providing further insight into the complexity of signaling networks.},

  doi          = {10.1111/mmi.14180},

  journal      = {Molecular microbiology},

  number       = 3,

  volume       = 111,

  place        = {United States},

  year         = {Sat Dec 08 00:00:00 EST 2018},

  month        = {Sat Dec 08 00:00:00 EST 2018}

}

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