Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography
Abstract
The practice of serial X-ray crystallography (SX) depends on efficient, continuous delivery of hydrated protein crystals while minimizing background scattering. Of the two major types of sample delivery devices, fixed-target devices offer several advantages over widely adopted jet injectors, including: lower sample consumption, clog-free delivery, and the ability to control on-chip crystal density to improve hit rates. In this study we present our development of versatile, inexpensive, and robust polymer microfluidic chips for routine and reliable room temperature serial measurements at both synchrotrons and X-ray free electron lasers (XFELs). Our design includes highly X-ray-transparent enclosing thin film layers tuned to minimize scatter background, adaptable sample flow layers tuned to match crystal size, and a large sample area compatible with both raster scanning and rotation based serial data collection. The optically transparent chips can be used both for in situ protein crystallization (to eliminate crystal handling) or crystal slurry loading, with prepared samples stable for weeks in a humidified environment and for several hours in ambient conditions. Serial oscillation crystallography, using a multi-crystal rotational data collection approach, at a microfocus synchrotron beamline (SSRL, beamline 12-1) was used to benchmark the performance of the chips. Furthermore, high-resolution structures (1.3–2.7 Å) were collectedmore »
- Authors:
-
[1];
[5];
[3];
[1]
- Univ. of California, Davis, CA (United States)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Lumen Bioscience, Seattle, WA (United States)
- Hauptman-Woodward Medical Research Institute, Buffalo, NY (United States)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Univ. of California, Davis, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); National Science Foundation (NSF); National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1884644
- Alternate Identifier(s):
- OSTI ID: 1832617
- Report Number(s):
- LLNL-JRNL-829465
Journal ID: ISSN 1473-0197; 1045356
- Grant/Contract Number:
- AC52-07NA27344; 1231306; R01GM117342; U19 AI144184; AC02-76SF00515; P30GM133894
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Lab on a Chip
- Additional Journal Information:
- Journal Volume: 21; Journal Issue: 24; Journal ID: ISSN 1473-0197
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Biological and medical sciences
Citation Formats
Gilbile, Deepshika, Shelby, Megan L., Lyubimov, Artem Y., Wierman, Jennifer L., Monteiro, Diana F., Cohen, Aina E., Russi, Silvia, Coleman, Matthew A., Frank, Matthias, and Kuhl, Tonya L. Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography. United States: N. p., 2021.
Web. doi:10.1039/d1lc00810b.
Gilbile, Deepshika, Shelby, Megan L., Lyubimov, Artem Y., Wierman, Jennifer L., Monteiro, Diana F., Cohen, Aina E., Russi, Silvia, Coleman, Matthew A., Frank, Matthias, & Kuhl, Tonya L. Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography. United States. https://doi.org/10.1039/d1lc00810b
Gilbile, Deepshika, Shelby, Megan L., Lyubimov, Artem Y., Wierman, Jennifer L., Monteiro, Diana F., Cohen, Aina E., Russi, Silvia, Coleman, Matthew A., Frank, Matthias, and Kuhl, Tonya L. Thu .
"Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography". United States. https://doi.org/10.1039/d1lc00810b. https://www.osti.gov/servlets/purl/1884644.
@article{osti_1884644,
title = {Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography},
author = {Gilbile, Deepshika and Shelby, Megan L. and Lyubimov, Artem Y. and Wierman, Jennifer L. and Monteiro, Diana F. and Cohen, Aina E. and Russi, Silvia and Coleman, Matthew A. and Frank, Matthias and Kuhl, Tonya L.},
abstractNote = {The practice of serial X-ray crystallography (SX) depends on efficient, continuous delivery of hydrated protein crystals while minimizing background scattering. Of the two major types of sample delivery devices, fixed-target devices offer several advantages over widely adopted jet injectors, including: lower sample consumption, clog-free delivery, and the ability to control on-chip crystal density to improve hit rates. In this study we present our development of versatile, inexpensive, and robust polymer microfluidic chips for routine and reliable room temperature serial measurements at both synchrotrons and X-ray free electron lasers (XFELs). Our design includes highly X-ray-transparent enclosing thin film layers tuned to minimize scatter background, adaptable sample flow layers tuned to match crystal size, and a large sample area compatible with both raster scanning and rotation based serial data collection. The optically transparent chips can be used both for in situ protein crystallization (to eliminate crystal handling) or crystal slurry loading, with prepared samples stable for weeks in a humidified environment and for several hours in ambient conditions. Serial oscillation crystallography, using a multi-crystal rotational data collection approach, at a microfocus synchrotron beamline (SSRL, beamline 12-1) was used to benchmark the performance of the chips. Furthermore, high-resolution structures (1.3–2.7 Å) were collected from five different proteins – hen egg white lysozyme, thaumatin, bovine liver catalase, concanavalin-A (type VI), and SARS-CoV-2 nonstructural protein NSP5. Overall, our modular fabrication approach enables precise control over the cross-section of materials in the X-ray beam path and facilitates chip adaption to different sample and beamline requirements for user-friendly, straightforward diffraction measurements at room temperature.},
doi = {10.1039/d1lc00810b},
journal = {Lab on a Chip},
number = 24,
volume = 21,
place = {United States},
year = {Thu Nov 25 00:00:00 EST 2021},
month = {Thu Nov 25 00:00:00 EST 2021}
}
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