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Title: Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography

We present a crystallography chip enabling in situ room temperature crystallography at microfocus synchrotron beamlines and X-ray free-electron laser (X-FEL) sources. Compared to other in situ approaches, we observe extremely low background and high diffraction data quality. The chip design is robust and allows fast and efficient loading of thousands of small crystals. The ability to load a large number of protein crystals, at room temperature and with high efficiency, into prescribed positions enables high throughput automated serial crystallography with microfocus synchrotron beamlines. In addition, we demonstrate the application of this chip for femtosecond time-resolved serial crystallography at the Linac Coherent Light Source (LCLS, Menlo Park, California, USA). As a result, the chip concept enables multiple images to be acquired from each crystal, allowing differential detection of changes in diffraction intensities in order to obtain high signal-to-noise and fully exploit the time resolution capabilities of XFELs.
Authors:
 [1] ;  [2] ;  [2] ;  [2] ;  [1] ;  [1] ;  [3] ;  [4] ;  [5] ;  [6] ;  [7] ;  [8] ;  [3] ;  [4] ;  [1] ;  [9]
  1. Univ. of Toronto, Toronto, ON (Canada)
  2. Max Planck Institute for the Structure and Dynamics of Matter, Hamburg (Germany)
  3. Rice Univ., Houston, TX (United States)
  4. National Institutes of Health, Bethesda, MD (United States)
  5. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  6. Diamond Light Source, Didcot (United Kingdom)
  7. Univ. of Toronto, Toronto, ON (Canada); Univ. of Toronto and Campbell Family Institute for Cancer Research, Univ. Health Network, Toronto, ON (Canada)
  8. Univ. of Hamburg, Hamburg (Germany)
  9. Univ. of Toronto, Toronto, ON (Canada); Max Planck Institute for the Structure and Dynamics of Matter, Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
Publication Date:
OSTI Identifier:
1230061
Grant/Contract Number:
AC03-76SF00515
Type:
Accepted Manuscript
Journal Name:
Structural Dynamics
Additional Journal Information:
Journal Volume: 2; Journal Issue: 5; Journal ID: ISSN 2329-7778
Publisher:
American Crystallographic Association/AIP
Research Org:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY X-ray diffraction; crystal structure; proteins; crystallization; time dependent material structure