Recombinant glucose uptake system
Abstract
Recombinant organisms are disclosed that contain a pathway for glucose uptake other than the pathway normally utilized by the host cell. In particular, the host cell is one in which glucose transport into the cell normally is coupled to PEP production. This host cell is transformed so that it uses an alternative pathway for glucose transport that is not coupled to PEP production. In a preferred embodiment, the host cell is a bacterium other than Z. mobilis that has been transformed to contain the glf and glk genes of Z. mobilis. By uncoupling glucose transport into the cell from PEP utilization, more PEP is produced for synthesis of products of commercial importance from a given quantity of biomass supplied to the host cells.
- Inventors:
-
- Gainesville, FL
- Groede, NL
- Delft, NL
- Issue Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- OSTI Identifier:
- 870823
- Patent Number(s):
- 5602030
- Assignee:
- University of Florida (Gainesville, FL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG05-86ER13574
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- recombinant; glucose; uptake; organisms; disclosed; contain; pathway; normally; utilized; host; cell; particular; transport; coupled; pep; production; transformed; alternative; preferred; embodiment; bacterium; z; mobilis; glf; glk; genes; uncoupling; utilization; produced; synthesis; products; commercial; importance; quantity; biomass; supplied; cells; preferred embodiment; host cells; host cell; recombinant organisms; glucose uptake; /435/
Citation Formats
Ingrahm, Lonnie O, Snoep, Jacob L, and Arfman, Nico. Recombinant glucose uptake system. United States: N. p., 1997.
Web.
Ingrahm, Lonnie O, Snoep, Jacob L, & Arfman, Nico. Recombinant glucose uptake system. United States.
Ingrahm, Lonnie O, Snoep, Jacob L, and Arfman, Nico. Wed .
"Recombinant glucose uptake system". United States. https://www.osti.gov/servlets/purl/870823.
@article{osti_870823,
title = {Recombinant glucose uptake system},
author = {Ingrahm, Lonnie O and Snoep, Jacob L and Arfman, Nico},
abstractNote = {Recombinant organisms are disclosed that contain a pathway for glucose uptake other than the pathway normally utilized by the host cell. In particular, the host cell is one in which glucose transport into the cell normally is coupled to PEP production. This host cell is transformed so that it uses an alternative pathway for glucose transport that is not coupled to PEP production. In a preferred embodiment, the host cell is a bacterium other than Z. mobilis that has been transformed to contain the glf and glk genes of Z. mobilis. By uncoupling glucose transport into the cell from PEP utilization, more PEP is produced for synthesis of products of commercial importance from a given quantity of biomass supplied to the host cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1997},
month = {Wed Jan 01 00:00:00 EST 1997}
}
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