Inhibition of potentially lethal radiation damage repair in normal and neoplastic human cells by 3-aminobenzamide: an inhibitor of poly(ADP-ribosylation)
The effect of 3-aminobenzamide (3AB), an inhibitor of poly(ADP-ribose) synthetase, on potentially lethal damage repair (PLDR) was investigated in normal human fibroblasts and four human tumor cell lines from tumors with varying degrees of radiocurability. The tumor lines selected were: Ewing's sarcoma, a bone tumor considered radiocurable and, human lung adenocarcinoma, osteosarcoma, and melanoma, three tumors considered nonradiocurable. PLDR was measured by comparing cell survival when cells were irradiated in a density-inhibited state and replated at appropriate cell numbers at specified times following irradiation to cell survival when cells were replated immediately following irradiation. 3AB was added to cultures 2 hr prior to irradiation and removed at the time of replating. Different test radiation doses were used for the various cell lines to obtain equivalent levels of cell survival. In the absence of inhibitor, PLDR was similar in all cell lines tested. In the presence of 8 mM 3AB, differential inhibition of PLDR was observed. PLDR was almost completely inhibited in Ewing's sarcoma cells and partially inhibited in normal fibroblast cells and osteosarcoma cells. No inhibition of PLDR was observed in the lung adenocarcinoma or melanoma cells. Except for the osteosarcoma cells, inhibition of PLDR by 3AB correlated well with radiocurability.
- Research Organization:
- Georgetown Univ. Medical Center, Washington, DC
- OSTI ID:
- 7023341
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 8
- Country of Publication:
- United States
- Language:
- English
Similar Records
3-Aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase, is a stimulator, not an inhibitor, of DNA repair
X-ray sensitivity of human tumor cells in vitro
Related Subjects
AMIDES
RADIOSENSITIVITY EFFECTS
OSTEOSARCOMAS
RADIOSENSITIVITY
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
FIBROBLASTS
IN VITRO
INHIBITION
NEOPLASMS
RADIOTHERAPY
SURVIVAL TIME
TUMOR CELLS
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CONNECTIVE TISSUE CELLS
DISEASES
MEDICINE
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION EFFECTS
RADIOLOGY
RECOVERY
REPAIR
SARCOMAS
SKELETAL DISEASES
SOMATIC CELLS
THERAPY
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture