3-Aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase, is a stimulator, not an inhibitor, of DNA repair

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(87)90385-5· OSTI ID:6521777

Cleaver, J E; Morgan, W F ^[1]

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1. Univ. of California, San Francisco (USA)

An inhibitor of poly(ADP-ribose) synthesis, 3-aminobenzamide (3AB), at low concentrations was found to reduce strand-break frequencies and increase repair replication in human lymphoid cells damaged by methyl methanoesulfonate. A concentration of 0.1 mM 3AB was adequate to produce a maximum effect on strand-break frequencies and repair replication. This evidence, together with previous measurements, demonstrates that 3AB cannot be regarded as an inhibitor of DNA repair; rather, it actually accelerates the ligation of DNA repair patches. Previous considerations of 3AB as a repair inhibitor may have derived from the use of excessive concentrations above 1 mM that may have stimulated additional damage and from the use of ethyl alcohol as a solvent for 3AB. Interpretations of the role of single-strand breaks and poly(ADP-ribose) in DNA repair, differentiation, and gene activity may need reevaluation because they have frequently been based on an erroneous notion of 3AB as a repair inhibitor, when its mode of action is, in fact, more complex.

• OSTI ID: 6521777
• Journal Information: Experimental Cell Research; (USA), Vol. 172:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
BIOLOGICAL EFFECTS
STRAND BREAKS
DNA REPAIR
CARBON 14 COMPOUNDS
ENZYME INHIBITORS
INHIBITION
LIGASES
METHYL METHANESULFONATE
NUCLEOTIDES
RADIOINDUCTION
RIBOSIDES
SCINTILLATION COUNTING
THYMIDINE
TRITIUM COMPOUNDS
X RADIATION
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
COUNTING TECHNIQUES
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IONIZING RADIATIONS
LABELLED COMPOUNDS
MUTAGENS
NUCLEOSIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
SULFONIC ACID ESTERS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture