Effect of inhibitors of poly(ADP-ribose)polymerase on the radiation response of HeLa S3 cells

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577628· OSTI ID:5543568

Burgman, P; Konings, A W ^[1]

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1. State Univ. Groningen (Netherlands)

The purpose of this study was to investigate possible involvement of poly(ADP-ribosyl)ation reactions in X-ray-induced cell killing, repair of potentially lethal damage (PLD), and formation and repair of radiation-induced DNA damage. As tools we used the inhibitors of poly(ADP-ribose)polymerase, 3-aminobenzamide (3AB), and 4-aminobenzamide (4AB). Both drugs inhibited PLD repair equally well but did not increase radiation-induced cell killing when cells were plated immediately after irradiation. 3AB affected repair of radiation-induced DNA damage, while 4AB had no effect. When 3AB was combined with aphidicolin (APC), it was found that the amount of DNA damage increased during the postirradiation incubation period. This means that the presence of 3AB stimulates the formation of DNA damage after X-irradiation. It is concluded that 3AB and 4AB sensitize HeLaS3 cells for radiation-induced cell killing by inhibiting repair of PLD. Because of the different effects of both inhibitors on repair of PLD and repair of radiation-induced DNA damage (a process known to be affected by inhibition of poly(ADP-ribosyl)ation), it is concluded that the observed inhibition of PLD repair is not caused by inhibition of poly(ADP-ribose)polymerase, and that the inhibitors affect repair of PLD and repair of DNA damage through independent mechanisms.

• OSTI ID: 5543568
• Journal Information: Radiation Research; (USA), Vol. 119:2; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ENZYME INHIBITORS
RADIOSENSITIVITY EFFECTS
HELA CELLS
DNA REPAIR
CELL KILLING
DNA
GENETIC RADIATION EFFECTS
INHIBITION
MAN
POLYMERASES
SURVIVAL CURVES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
ENZYMES
GENETIC EFFECTS
MAMMALS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
RADIATION EFFECTS
RECOVERY
REPAIR
TRANSFERASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture