Novel methods for physical mapping of the human genome applied to the long arm of chromosome 5: Progress report for the period June 1, 1988 to October 1, 1988

Technical Report · Sat Oct 01 00:00:00 EDT 1988

OSTI ID:6786763

McClelland, M; Westbrook, C

The linkage of two genes IL-4 and IL-5 has been determined in the region of interest. These genes lie within 310,000 base pairs of each other, as determined by PFE. This is a contribution to our long term goal of producing a detailed physical map of this region. We have reduced to practice two methylase/DpnI strategies, with the specificities TCGATCGA and ATCGATCGAT. In addition, we have determined that cloned M.Xbal methylates the correct TCTAG/sup m6/A sequence for use in a new twelve base pair TCTAGATCTAGA cleavage specificity. These cleavage systems are being applied to a somatic cell hybrid that contains part of human 5q.

• Research Organization: Illinois Univ., Chicago (USA)
• DOE Contract Number: FG02-88ER60690
• OSTI ID: 6786763
• Report Number(s): DOE/ER/60690-1; ON: DE89005820
• Resource Relation: Other Information: Paper copy only, copy does not permit microfiche production
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
GENETIC MAPPING
DNA-CLONING
HUMAN POPULATIONS
LEUKEMIA
MUTATIONS
PROGRESS REPORT
RADIATION INJURIES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CLONING
DISEASES
DNA HYBRIDIZATION
DOCUMENT TYPES
HEMIC DISEASES
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
INJURIES
MAPPING
NEOPLASMS
POPULATIONS
RADIATION EFFECTS
550200* - Biochemistry
550400 - Genetics