Novel methods for physical mapping of the human genome applied to the long arm of chromosome 5
Our objective was to develop novel methods for mapping of the human genome. The techniques to assessed were: (1) three methods for the production of unique sequence clones from the region of interest; (2) novel methods for the production and separation of multi-megabase DNA fragments; (3) methods for the production of physical linking clones'' that contain rare restriction sites; (4) application of these methods and available resources to map the region of interest. We have also concentrated on developing rare-cleavage tools based on restriction enzymes and methylases. In the last year we: studied the effect of methylation on enzymes used for genome mapping; collaborated on characterization of two new isoschizomers; developed a reliable way to produce partial digests of DNA in agarose and applied it to the human genome; and applied a method to double the apparent specificity of rare-cutter'' endonucleases. 35 refs., 1 tab.
- Research Organization:
- California Inst. of Biological Research, La Jolla, CA (USA)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- FG03-89ER60913
- OSTI ID:
- 5644269
- Report Number(s):
- DOE/ER/60913-T1; ON: DE91012894
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENETIC MAPPING
RESEARCH PROGRAMS
HUMAN CHROMOSOME 5
BACILLUS
BIOLOGICAL MARKERS
DNA
DNA METHYLASES
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
ENDONUCLEASES
ESCHERICHIA COLI
METHYLATION
MICE
NUCLEOTIDES
PROGRESS REPORT
PURIFICATION
SALMONELLA
TRANSFER RNA
ANIMALS
BACTERIA
CHEMICAL REACTIONS
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DNA-ASE
DOCUMENT TYPES
ENZYMES
ESTERASES
HYBRIDIZATION
HYDROLASES
LYASES
MAMMALS
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PROTEINS
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry
550400 - Genetics