Novel methods for physical mapping of the human genome applied to the long arm of chromosome 5

Technical Report · Sun Dec 01 00:00:00 EST 1991

DOI:https://doi.org/10.2172/7206400· OSTI ID:7206400

McClelland, M

The object of our current grant is to develop novel methods for mapping of the human genome. The techniques to be assessed were: (1) three methods for the production of unique sequence clones from the region of interest; (2) novel methods for the production and separation of multi-megabase DNA fragments; (3) methods for the production of physical linking clones'' that contain rare restriction sites; (4) application of these methods and available resources to map the region of interest. Progress includes: In the first two years methods were developed for physical mapping and the production of arrayed clones; We have concentrated on developing rare- cleavage tools based or restriction endonucleases and methylases; We studied the effect of methylation on enzymes used for PFE mapping of the human genome; we characterized two new isoschizomers of rare cutting endonucleases; we developed a reliable way to produce partial digests of DNA in agarose plugs and applied it to the human genome; and we applied a method to double the apparent specificity of the rare-cutter'' endonucleases.

• Research Organization: California Inst. of Biological Research, La Jolla, CA (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG03-89ER60913
• OSTI ID: 7206400
• Report Number(s): DOE/ER/60913-4; ON: DE92016055
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENDONUCLEASES
BIOCHEMICAL REACTION KINETICS
GENETIC MAPPING
OPTIMIZATION
HUMAN CHROMOSOME 5
DNA METHYLASES
ENZYME ACTIVITY
PROGRESS REPORT
CHROMOSOMES
DNA-ASE
DOCUMENT TYPES
ENZYMES
ESTERASES
HUMAN CHROMOSOMES
HYDROLASES
KINETICS
LYASES
MAPPING
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PROTEINS
REACTION KINETICS
550400* - Genetics
550200 - Biochemistry