Microsecond Microfluidic Mixing for Investigation of Protein Folding Kinetics
Conference
·
OSTI ID:15014683
We have developed and characterized a mixer to study the reaction kinetics of protein folding on a microsecond timescale. The mixer uses hydrodynamic focusing of pressure-driven flow in a microfluidic channel to reduce diffusion times as first demonstrated by Knight et al.[1]. Features of the mixer include 1 {micro}s mixing times, sample consumptions of order 1 nl/s, loading sample volumes on the order of microliters, and the ability to manufacture in fused silica for compatibility with most spectroscopic methods.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 15014683
- Report Number(s):
- UCRL-PROC-209712; TRN: US200807%%700
- Resource Relation:
- Conference: Presented at: Micro Total Analysis Systems, Squaw Valley, CA (US), 10/05/2003--10/09/2003; Other Information: PBD: 10 Feb 2005
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:15014683