Crystal structures of sialyltransferase from Photobacterium damselae

Huynh, Nhung; Li, Yanhong; Yu, Hai; Huang, Shengshu; Lau, Kam; Chen, Xi; Fisher, Andrew J.

doi:10.1016/j.febslet.2014.11.003

Title: Crystal structures of sialyltransferase from Photobacterium damselae

Journal Article · Sat Nov 15 00:00:00 EST 2014 · FEBS Letters

DOI:https://doi.org/10.1016/j.febslet.2014.11.003· OSTI ID:1347143

Huynh, Nhung ^[1]; Li, Yanhong ^[1]; Yu, Hai ^[1]; Huang, Shengshu ^[1]; Lau, Kam ^[1]; Chen, Xi ^[1]; Fisher, Andrew J. ^[1]

Univ. of California, Davis, CA (United States)

Sialyltransferase structures fall into either GT-A or GT-B glycosyltransferase fold. Some sialyltransferases from the Photobacterium genus have been shown to contain an additional N-terminal immunoglobulin (Ig)-like domain. Photobacterium damselae α2–6-sialyltransferase has been used efficiently in enzymatic and chemoenzymatic synthesis of α2–6-linked sialosides. In this paper, we report three crystal structures of this enzyme. Two structures with and without a donor substrate analog CMP-3F(a)Neu5Ac contain an immunoglobulin (Ig)-like domain and adopt the GT-B sialyltransferase fold. The binary structure reveals a non-productive pre-Michaelis complex, which are caused by crystal lattice contacts that prevent the large conformational changes. The third structure lacks the Ig-domain. Finally, comparison of the three structures reveals small inherent flexibility between the two Rossmann-like domains of the GT-B fold.

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Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Inst. of Health (NIH) (United States)

Grant/Contract Number:: R01HD065122; R01GM094523; P41GM103393; P41RR001209

OSTI ID:: 1347143

Alternate ID(s):: OSTI ID: 1227562

Journal Information:: FEBS Letters, Vol. 588, Issue 24; ISSN 0014-5793

Publisher:: Federation of European Biochemical SocietiesCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 19 works

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59 BASIC BIOLOGICAL SCIENCES
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protein crystal structure
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CMP-3F(a)Neu5Ac
Photobacterium damselae

Title: Crystal structures of sialyltransferase from Photobacterium damselae

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