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Title: Hydrogenase polypeptide and methods of use

Patent ·
OSTI ID:1237279

Provided herein are polypeptides having hydrogenase activity. The polypeptide may be multimeric, and may have hydrogenase activity of at least 0.05 micromoles H.sub.2 produced min.sup.-1 mg protein.sup.-1. Also provided herein are polynucleotides encoding the polypeptides, genetically modified microbes that include polynucleotides encoding one or more subunits of the multimeric polypeptide, and methods for making and using the polypeptides.

Research Organization:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-05ER15710
Assignee:
University of Georgia Research Foundation, Inc.
Patent Number(s):
9,249,440
Application Number:
14/204,802
OSTI ID:
1237279
Resource Relation:
Patent File Date: 2014 Mar 11
Country of Publication:
United States
Language:
English

References (67)

Cloning and Expression inEscherichia coliof the Recombinant His-Tagged DNA Polymerases fromPyrococcus furiosusandPyrococcus woesei journal October 1998
General vectors for archaeal hyperthermophiles: Strategies based on a mobile intron and a plasmid journal May 1996
Hydrogenase journal December 1980
The structure and mechanism of iron-hydrogenases journal November 1990
Nickel hydrogenases: in search of the active site journal December 1994
Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins journal July 2006
Infrared-Detectable Group Senses Changes in Charge Density on the Nickel Center in Hydrogenase from Chromatium vinosum journal April 1995
Generation of New Hydrogen-Recycling Rhizobiaceae Strains by Introduction of a Novel hup Minitransposon journal October 2000
Maturation of [NiFe]-hydrogenases in Escherichia coli: The HypC Cycle journal November 2002
Catalytic partial oxidation of natural gas to syngas journal April 1995
Maturation of Hydrogenases book September 2006
Deciphering the message in protein sequences: tolerance to amino acid substitutions journal March 1990
[Fe 2 (SR) 2 (μ-CO)(CNMe) 6 ] 2+ and Analogues:  A New Class of Diiron Dithiolates as Structural Models for the H o x A ir State of the Fe-Only Hydrogenase journal November 2004
Infrared Studies of the CO-Inhibited Form of the Fe-Only Hydrogenase from Clostridium pasteurianum I:  Examination of Its Light Sensitivity at Cryogenic Temperatures journal February 2002
Thermotolerant Hydrogenases: Biological Diversity, Properties, and Biotechnological Applications journal January 2008
Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C journal June 1986
Molecular Biology of Hydrogen Utilization in Aerobic Chemolithotrophs journal October 1993
Structural bases for the catalytic mechanism of [NiFe] hydrogenases journal August 1998
Review of fuel processing catalysts for hydrogen production in PEM fuel cell systems journal October 2002
Biological activition of hydrogen journal January 1997
Large-scale applications of NAD(P)-dependent oxidoreductases: recent developments journal December 1999
Stability and sulfur-reduction activity in non-aqueous phase liquids of the hydrogenase from the hyperthermophile Pyrococcus furiosus journal October 1999
Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System journal March 2006
Requirements for Heterologous Production of a Complex Metalloenzyme: the Membrane-Bound [NiFe] Hydrogenase journal September 2005
Carbon Monoxide as an Intrinsic Ligand to Iron in the Active Site of the Iron−Sulfur-Cluster-Free Hydrogenase H 2 -Forming Methylenetetrahydromethanopterin Dehydrogenase As Revealed by Infrared Spectroscopy journal November 2004
[18] Hydrogenases I and II from Pyrococcus furiosus book January 2001
Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus : A key role for NADPH journal October 1994
Enhanced hydrogen production from glucose by metabolically engineered Escherichia coli journal October 2007
Inhibition of hydrogen uptake in Escherichia coli by expressing the hydrogenase from the cyanobacterium Synechocystis sp. PCC 6803 journal January 2007
Escherichia coli hydrogenase 3 is a reversible enzyme possessing hydrogen uptake and synthesis activities journal August 2007
[FeFe] hydrogenases and their evolution: a genomic perspective journal March 2007
Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of hydrogen producing Enterobacter cloacae IIT-BT 08 journal November 2004
Overexpression of a hydrogenase gene in Clostridium paraputrificum to enhance hydrogen gas production journal May 2005
Fe-only hydrogenases: structure, function and evolution journal July 2002
Crystallographic and FTIR Spectroscopic Evidence of Changes in Fe Coordination Upon Reduction of the Active Site of the Fe-Only Hydrogenase from Desulfovibrio d esulfuricans journal February 2001
A novel FeS cluster in Fe-only hydrogenases journal March 2000
Desulfovibrio desulfuricans iron hydrogenase: the structure shows unusual coordination to an active site Fe binuclear center journal January 1999
Carbamoylphosphate requirement for synthesis of the active center of [NiFe]-hydrogenases journal January 2001
Characterization of the locus encoding the [Ni-Fe] sulfhydrogenase from the archaeon Pyrococcus furiosus: evidence for a relationship to bacterial sulfite reductases journal February 1995
X-ray Crystal Structure of the Fe-Only Hydrogenase (CpI) from Clostridium pasteurianum to 1.8 Angstrom Resolution journal December 1998
Carbon Monoxide and Cyanide as Intrinsic Ligands to Iron in the Active Site of [NiFe]-Hydrogenases: NiFe(CN) journal February 1999
Expression of a functional NAD-reducing [NiFe] hydrogenase from the gram-positive Rhodococcus opacus in the gram-negative Ralstonia eutropha journal November 2001
Discovery of Two Novel Radical S -Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase journal April 2004
Structure-function relationships among the nickel-containing hydrogenases journal February 1992
Development of gfp vectors for expression in Listeria monocytogenes and other low G+C gram positive bacteria journal May 2001
Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization journal June 1991
A simple energy-conserving system: Proton reduction coupled to proton translocation journal June 2003
Applications of a peptide ligand for streptavidin: the Strep-tag journal December 1999
Protein production by auto-induction in high-density shaking cultures journal May 2005
BLAST 2 Sequences, a new tool for comparing protein and nucleotide sequences journal May 1999
Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems journal June 2006
De Novo Design of Synthetic Di-Iron(I) Complexes as Structural Models of the Reduced Form of Iron−Iron Hydrogenase journal February 2006
Classification and phylogeny of hydrogenases journal August 2001
Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview journal October 2007
Structure of the [NiFe] Hydrogenase Active Site:  Evidence for Biologically Uncommon Fe Ligands journal January 1996
The active site and catalytic mechanism of NiFe hydrogenases journal January 2003
In vitro hydrogen production by glucose dehydrogenase and hydrogenase journal July 1996
High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway journal May 2007
Theoretical Studies of [FeFe]-Hydrogenase:  Infrared Fingerprints of the Dithiol-Bridging Ligand in the Active Site journal February 2007
Effect of nickel on activity and subunit composition of purified hydrogenase from Nocardia opaca 1b journal February 1984
Conjugal transfer of hydrogen-oxidizing ability of Alcaligeneshydrogenophilus to Pseudomonasoxalaticus journal May 1986
Isolation of hydrogen-oxidation gene from Alcaligeneshydrogenophilus and its expression in Pseudomonasoxalaticus journal May 1986
EPR and M�ssbauer spectroscopic studies on the tetrameric, NAD-linked hydrogenase of Nocardia opaca 1b and its two dimers: 1. The ??-dimer?a prototype of a simple hydrogenase journal April 1995
Synthesis of protein with an identification peptide patent October 1987
Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same patent November 1988
Process of purifying recombinant proteins and compounds useful in such process patent January 1997
Protein expression system patent August 1999