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Title: Localization of polyhydroxybutyrate in sugarcane using Fourier-transform infrared microspectroscopy and multivariate imaging

Journal Article · · Biotechnology for Biofuels
 [1];  [2];  [3];  [4];  [2];  [5];  [6]
  1. Univ. of Queensland, Queensland (Australia); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sage Analytics, Boulder, CO (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sandia National Lab., Livermore, CA (United States)
  4. Univ. of Queensland, Queensland (Australia); Univ. of North Texas, Denton, TX (United States)
  5. Univ. of Queensland, Queensland (Australia); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sandia National Lab., Livermore, CA (United States)
  6. Univ. of Queensland, Queensland (Australia)
+ Show Author Affiliations

Slow-degrading, fossil fuel-derived plastics can have deleterious effects on the environment, especially marine ecosystems. The production of bio-based, biodegradable plastics from or in plants can assist in supplanting those manufactured using fossil fuels. Polyhydroxybutyrate (PHB) is one such biodegradable polyester that has been evaluated as a possible candidate for relinquishing the use of environmentally harmful plastics. Results: PHB, possessing similar properties to polyesters produced from non-renewable sources, has been previously engineered in sugarcane, thereby creating a high-value co-product in addition to the high biomass yield. This manuscript illustrates the coupling of a Fourier-transform infrared microspectrometer, equipped with a focal plane array (FPA) detector, with multivariate imaging to successfully identify and localize PHB aggregates. Principal component analysis imaging facilitated the mining of the abundant quantity of spectral data acquired using the FPA for distinct PHB vibrational modes. PHB was measured in the chloroplasts of mesophyll and bundle sheath cells, acquiescent with previously evaluated plant samples. Conclusion: This study demonstrates the power of IR microspectroscopy to rapidly image plant sections to provide a snapshot of the chemical composition of the cell. While PHB was localized in sugarcane, this method is readily transferable to other value-added co-products in different plants.

Research Organization:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23), Biological Systems Science Division (SC-23.2 )
Grant/Contract Number:
AC04-94AL85000; AC02-05CH11231
OSTI ID:
1213399
Alternate ID(s):
OSTI ID: 1512216
Journal Information:
Biotechnology for Biofuels, Vol. 8, Issue 1; ISSN 1754-6834
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 10 works
Citation information provided by
Web of Science

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Monitoring Chemical Changes on the Surface of Kenaf Fiber during Degumming Process Using Infrared Microspectroscopy journal April 2017

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Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
36 MATERIALS SCIENCE
infrared imaging
focal plane array
polyhydroxybutyrate
sugarcane
multivariate imaging