Crystal structure of the Alcanivorax borkumensis YdaH transporter reveals an unusual topology

Bolla, Jani Reddy; Su, Chih-Chia; Delmar, Jared A.; Radhakrishnan, Abhijith; Kumar, Nitin; Chou, Tsung-Han; Long, Feng; Rajashankar, Kanagalaghatta R.; Yu, Edward W.

doi:10.1038/ncomms7874

Title: Crystal structure of the Alcanivorax borkumensis YdaH transporter reveals an unusual topology

Journal Article · Mon Apr 20 00:00:00 EDT 2015 · Nature Communications

DOI:https://doi.org/10.1038/ncomms7874· OSTI ID:1178476

Bolla, Jani Reddy ^[1]; Su, Chih-Chia ^[2]; Delmar, Jared A. ^[2]; Radhakrishnan, Abhijith ^[1]; Kumar, Nitin ^[1]; Chou, Tsung-Han ^[2]; Long, Feng ^[2]; Rajashankar, Kanagalaghatta R. ^[3]; Yu, Edward W. ^[4]

Iowa State Univ., IA (United States). Dept. of Chemistry
Iowa State Univ., IA (United States). Dept. of Physics and Astronomy
Argonne National Laboratory, IL (United States). NE-CAT and Dept. of chemistry and Chemical BIology
Iowa State Univ., IA (United States). Dept. of Chemistry and Dept. of Physics and Astronomy

The potential of the folic acid biosynthesis pathway as a target for the development of antibiotics has been clinically validated. However, many pathogens have developed resistance to these antibiotics, prompting a re-evaluation of potential drug targets within the pathway. The ydaH gene of Alcanivorax borkumensis encodes an integral membrane protein of the AbgT family of transporters for which no structural information was available. Here we report the crystal structure of A. borkumensis YdaH, revealing a dimeric molecule with an architecture distinct from other families of transporters. YdaH is a bowl-shaped dimer with a solvent-filled basin extending from the cytoplasm to halfway across the membrane bilayer. Each subunit of the transporter contains nine transmembrane helices and two hairpins that suggest a plausible pathway for substrate transport. Further analyses also suggest that YdaH could act as an antibiotic efflux pump and mediate bacterial resistance to sulfonamide antimetabolite drugs.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1178476

Journal Information:: Nature Communications, Vol. 6; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 30 works

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