Expression of SRY transcripts in preimplantation human embryos
Journal Article
·
· American Journal of Medical Genetics
- Northwestern Univ. Medical School, Chicago, IL (United States); and others
We have examined the expression of SRY mRNA in individual in vitro fertilized preimplantation human embryos; because of ethical constraints, these studies were confined to embryos with one and three pronuclei. Using a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) assay, we observed SRY mRNA at the one-cell through the blastula stages but not in spermatoza. These results indicate that the de novo transcription of this sex-specific gene occurs at a developmental time considerably earlier than that of gonadal differentiation. Our results also indicate that in vitro fertilized embryos with one pronucleus are likely to be diploid. 39 refs., 1 fig., 2 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 91104
- Journal Information:
- American Journal of Medical Genetics, Vol. 55, Issue 1; Other Information: PBD: 2 Jan 1995
- Country of Publication:
- United States
- Language:
- English
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