Evaluation of a GFP Report Gene Construct for Environmental Arsenic Detection
Detection of arsenic and other heavy metal contaminants in the environment is critical to ensuring safe drinking water and effective cleanup of historic activities that have led to widespread contamination of soil and groundwater. Biosensors have the potential to significantly reduce the costs associated with site characterization and long term environmental monitoring. By exploiting the highly selective and sensitive natural mechanisms by which bacteria and other living organisms respond to heavy metals, and fusing transcriptionally active components of these mechanisms to reporter genes, such as B-galactosidase, bacterial luciferase (lux), or green fluorescent protein (GFP) from marine jellyfish, it is possible to produce inexpensive, yet effective biosensors. This article describes the response to submicrogram quantities of arsenite and arsenate of a whole cell arsenic biosensor utilizing a GFP reporter gene.
- Research Organization:
- Idaho National Lab. (INL), Idaho Falls, ID (United States)
- Sponsoring Organization:
- USDOE Office of Environmental Management (EM) (US)
- DOE Contract Number:
- AC07-99ID13727
- OSTI ID:
- 804077
- Report Number(s):
- INEEL/JOU-02-00447; TLNTA2; TRN: US200310%%70
- Journal Information:
- Talanta, Other Information: PBD: 28 Mar 2002; ISSN 0039-9140
- Country of Publication:
- United States
- Language:
- English
Similar Records
ATP-dependent arsenical pumps, gene products of the arsenical resistance operon of R-factor R773
Population differences in the human arsenic (+ 3 oxidation state) methyltransferase (AS3MT) gene polymorphism detected by using genotyping method