Continuous separation of proteins by annular chromatography
Abstract
In this paper, the separation of protein mixtures by continuous annular chromatography (CAC) is studied in a preparative-scale apparatus. S-Sepharose, a strong-acid porous cation-exchange resin is used as the separation medium, and mixtures of albumin, hemoglobin and cytochrome c are used as model separation system. Equilibrium and mass-transfer parameters are developed for this system on the basis of fixed-bed chromatograph experiments. A mathematical model is then successfully used in conjunction with these parameters to simulate the performance of the CAC separations. The continuous separation performance of the annular apparatus is found to be essentially the same as the batchwise performance of an equivalent conventional chromatograph, making the unit attractive for preparative and process-scale applications where continuous throughput is desirable.
- Authors:
-
- Dept. of Chemical Engineering, Univ. of Virginia, Charlottesville, VA (US)
- Chemical Technology Div., Oak Ridge National Lab., Oak Ridge, TN (US)
- Publication Date:
- OSTI Identifier:
- 7279431
- Resource Type:
- Journal Article
- Journal Name:
- Industrial and Engineering Chemistry Research; (United States)
- Additional Journal Information:
- Journal Volume: 30:5; Journal ID: ISSN 0888-5885
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; PROTEINS; SEPARATION PROCESSES; ALBUMINS; CATIONS; CHROMATOGRAPHY; CYTOCHROMES; HEMOGLOBIN; ION EXCHANGE; MASS TRANSFER; MATHEMATICAL MODELS; MIXTURES; RESINS; CARBOXYLIC ACIDS; CHARGED PARTICLES; DISPERSIONS; GLOBINS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; IONS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC POLYMERS; PETROCHEMICALS; PETROLEUM PRODUCTS; PIGMENTS; POLYMERS; PORPHYRINS; 400105* - Separation Procedures; 550200 - Biochemistry
Citation Formats
Bloomingburg, G F, Bauer, J S, Carta, G, and Byers, C H. Continuous separation of proteins by annular chromatography. United States: N. p., 1991.
Web. doi:10.1021/ie00053a031.
Bloomingburg, G F, Bauer, J S, Carta, G, & Byers, C H. Continuous separation of proteins by annular chromatography. United States. https://doi.org/10.1021/ie00053a031
Bloomingburg, G F, Bauer, J S, Carta, G, and Byers, C H. 1991.
"Continuous separation of proteins by annular chromatography". United States. https://doi.org/10.1021/ie00053a031.
@article{osti_7279431,
title = {Continuous separation of proteins by annular chromatography},
author = {Bloomingburg, G F and Bauer, J S and Carta, G and Byers, C H},
abstractNote = {In this paper, the separation of protein mixtures by continuous annular chromatography (CAC) is studied in a preparative-scale apparatus. S-Sepharose, a strong-acid porous cation-exchange resin is used as the separation medium, and mixtures of albumin, hemoglobin and cytochrome c are used as model separation system. Equilibrium and mass-transfer parameters are developed for this system on the basis of fixed-bed chromatograph experiments. A mathematical model is then successfully used in conjunction with these parameters to simulate the performance of the CAC separations. The continuous separation performance of the annular apparatus is found to be essentially the same as the batchwise performance of an equivalent conventional chromatograph, making the unit attractive for preparative and process-scale applications where continuous throughput is desirable.},
doi = {10.1021/ie00053a031},
url = {https://www.osti.gov/biblio/7279431},
journal = {Industrial and Engineering Chemistry Research; (United States)},
issn = {0888-5885},
number = ,
volume = 30:5,
place = {United States},
year = {Wed May 01 00:00:00 EDT 1991},
month = {Wed May 01 00:00:00 EDT 1991}
}