Continuous separation of proteins by annular chromatography
- Dept. of Chemical Engineering, Univ. of Virginia, Charlottesville, VA (US)
- Chemical Technology Div., Oak Ridge National Lab., Oak Ridge, TN (US)
In this paper, the separation of protein mixtures by continuous annular chromatography (CAC) is studied in a preparative-scale apparatus. S-Sepharose, a strong-acid porous cation-exchange resin is used as the separation medium, and mixtures of albumin, hemoglobin and cytochrome c are used as model separation system. Equilibrium and mass-transfer parameters are developed for this system on the basis of fixed-bed chromatograph experiments. A mathematical model is then successfully used in conjunction with these parameters to simulate the performance of the CAC separations. The continuous separation performance of the annular apparatus is found to be essentially the same as the batchwise performance of an equivalent conventional chromatograph, making the unit attractive for preparative and process-scale applications where continuous throughput is desirable.
- OSTI ID:
- 7279431
- Journal Information:
- Industrial and Engineering Chemistry Research; (United States), Vol. 30:5; ISSN 0888-5885
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
PROTEINS
SEPARATION PROCESSES
ALBUMINS
CATIONS
CHROMATOGRAPHY
CYTOCHROMES
HEMOGLOBIN
ION EXCHANGE
MASS TRANSFER
MATHEMATICAL MODELS
MIXTURES
RESINS
CARBOXYLIC ACIDS
CHARGED PARTICLES
DISPERSIONS
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
PIGMENTS
POLYMERS
PORPHYRINS
400105* - Separation Procedures
550200 - Biochemistry