Tissue distribution of sup 3 H-nicotine in rats after bolus or constant injection

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Proceedings of the Society for Experimental Biology and Medicine; (USA)

OSTI ID:7266890

Chowdhury, P; Pasley, J N; Rayford, P L ^[1]

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1. Univ. of Arkansas for Medical Sciences, Little Rock (USA)

Two groups of rats, (N = 7), were fasted for 24 hrs prior to the study. On the day of the experiment, the animals were anesthetized and infused with either 5 ml nicotine solution (200 {mu}g/L) in saline containing 5 {mu}c {sup 3}H-nicotine, (sp. activity 50-80 mCi/mol) for 90 minutes or injected as a bolus with 0.5 ml of the same nicotine (200 {mu}g/L) solution. The animals were sacrificed 60 minutes after the injection or after the infusion was stopped. Blood and tissue samples were counted by liquid scintillation counting. Percent distribution of {sup 3}H-nicotine per gm of tissue was calculated from the total radioactivity recovered in individual tissues over the total activity injected into the rat and the values were compared using student's t test. Results: Distribution of {sup 3}H-nicotine was found highest in kidney (45-49%) among all tissues examined and was not different between routes of administration. Significantly higher retention of {sup 3}H-nicotine was found with continuous infusion in esophagus, fundus, antrum, spleen, cecum, pancreas, testes, heart and muscle when {sup 3}H-nicotine retentions were compared with bolus injection. In contrast, the distribution of {sup 3}H-nicotine in adrenal gland, was significantly lower in continuous infusion group. Distribution in blood was 6 fold higher in continuous infusion (7.26%) compared to bolus (1.11%) injection. The distribution {sup 3}H-nicotine in other tissues were not different by either routes of injection.

• OSTI ID: 7266890
• Journal Information: Proceedings of the Society for Experimental Biology and Medicine; (USA), Vol. 191:1; ISSN 0037-9727
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NICOTINE
TISSUE DISTRIBUTION
ADRENAL GLANDS
BLOOD
GASTROINTESTINAL TRACT
HEART
KIDNEYS
MUSCLES
RATS
RETENTION
SCINTILLATION COUNTING
SPLEEN
TESTES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALOIDS
AMINES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
AZOLES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
COUNTING TECHNIQUES
DIGESTIVE SYSTEM
DISTRIBUTION
DRUGS
ENDOCRINE GLANDS
GLANDS
GONADS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MALE GENITALS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PYRIDINES
PYRROLES
PYRROLIDINES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques