Calcium mobilization and phosphoinositide turnover in fluoride-activated human neutrophils
Fluoride ion, at concentrations above 10 mM, has been found to activate a superoxide production response in human neutrophils which is strongly dependent on the presence of extracellular calcium. In an attempt to further explore the calcium requirement of fluoride-induced neutrophil activation, intracellular calcium concentrations were monitored through use of the fluorescent calcium probe, Quin 2. Fluoride ion, at concentrations between 10 and 20 mM, was found to elicit a rise in intracellular calcium levels which was characterized by a lag period of 4 to 10 min and a prolonged duration of action (greater than 20 min). In contrast, the chemotactic peptide, formylmethionyl-leucyl-phenylalanine (FMLP), induced a rise in intracellular calcium concentration which peaked within 1 min. Preincubation of cells with 1 ..mu..g/ml pertussis toxin resulted in inhibition of the FMLP-induced response, but not that elicited by fluoride. Furthermore, anion exchange chromatography indicated that inositol phosphate accumulation occurred in fluoride-treated cells in association with calcium mobilization. Recent evidence suggests that the FMLP receptor is coupled to phospholipase C and phosphoinositide turnover through a guanine nucleotide binding protein susceptible to inhibition by pertussis toxin. Present results suggest that fluoride ion may serve to activate this protein in a manner resistant to inhibition by pertussis toxin.
- Research Organization:
- Univ. of Alberta, Edmonton
- OSTI ID:
- 7245098
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
FLUORIDES
BIOLOGICAL EFFECTS
PHOSPHOLIPIDS
BIOCHEMICAL REACTION KINETICS
FLUORESCENCE
INHIBITION
INOSITOL
ION EXCHANGE CHROMATOGRAPHY
MAN
NEUTROPHILS
PHOSPHATES
PROTEINS
TOXINS
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CHROMATOGRAPHY
ESTERS
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
INOSITOLS
KINETICS
LEUKOCYTES
LIPIDS
LUMINESCENCE
MAMMALS
MATERIALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PRIMATES
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
TOXIC MATERIALS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology