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Title: Calcium mobilization and phosphoinositide turnover in fluoride-activated human neutrophils

Fluoride ion, at concentrations above 10 mM, has been found to activate a superoxide production response in human neutrophils which is strongly dependent on the presence of extracellular calcium. In an attempt to further explore the calcium requirement of fluoride-induced neutrophil activation, intracellular calcium concentrations were monitored through use of the fluorescent calcium probe, Quin 2. Fluoride ion, at concentrations between 10 and 20 mM, was found to elicit a rise in intracellular calcium levels which was characterized by a lag period of 4 to 10 min and a prolonged duration of action (greater than 20 min). In contrast, the chemotactic peptide, formylmethionyl-leucyl-phenylalanine (FMLP), induced a rise in intracellular calcium concentration which peaked within 1 min. Preincubation of cells with 1 ..mu..g/ml pertussis toxin resulted in inhibition of the FMLP-induced response, but not that elicited by fluoride. Furthermore, anion exchange chromatography indicated that inositol phosphate accumulation occurred in fluoride-treated cells in association with calcium mobilization. Recent evidence suggests that the FMLP receptor is coupled to phospholipase C and phosphoinositide turnover through a guanine nucleotide binding protein susceptible to inhibition by pertussis toxin. Present results suggest that fluoride ion may serve to activate this protein in a manner resistant to inhibitionmore » by pertussis toxin.« less
Authors:
;
Publication Date:
OSTI Identifier:
7245098
Report Number(s):
CONF-8606151-
Journal ID: CODEN: FEPRA
Resource Type:
Conference
Resource Relation:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
Research Org:
Univ. of Alberta, Edmonton
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CALCIUM COMPOUNDS; MEMBRANE TRANSPORT; FLUORIDES; BIOLOGICAL EFFECTS; PHOSPHOLIPIDS; BIOCHEMICAL REACTION KINETICS; FLUORESCENCE; INHIBITION; INOSITOL; ION EXCHANGE CHROMATOGRAPHY; MAN; NEUTROPHILS; PHOSPHATES; PROTEINS; TOXINS; ALKALINE EARTH METAL COMPOUNDS; ANIMALS; ANTIGENS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; CHROMATOGRAPHY; ESTERS; FLUORINE COMPOUNDS; HALIDES; HALOGEN COMPOUNDS; INOSITOLS; KINETICS; LEUKOCYTES; LIPIDS; LUMINESCENCE; MAMMALS; MATERIALS; MONOSACCHARIDES; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PRIMATES; REACTION KINETICS; SACCHARIDES; SEPARATION PROCESSES; TOXIC MATERIALS; VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology