Demonstration of paternal inheritance of plastids in Picea (Pinaceae). [Hybridization of cloned, sup 32 -P labeled, petunia cpDNA]

Miscellaneous · Fri Jan 01 00:00:00 EST 1988

OSTI ID:7194608

Stine, M

Chloroplast DNA (cpDNA) was purified from Picea glauca, P. pungens, P. engelmannii, and P. omorika, and was digested with several restriction endonucleases. Interspecific restriction fragment length polymorphisms (RFLPs) of cpDNA were identified. The RFLPs were identified as cpDNA by the hybridization of cloned, {sup 32}-P labeled, petunia cpDNA to the polymorphic bands, and by the lack of hybridization of a cloned and labeled mtDNA probe from maize. Chloroplast DNA RFLPs that showed no intraspecific variation when examined across the natural range for each species, were used as markers to follow the inheritance of plastids in interspecific hybrids. The inheritance of plastids was determined for F{sub 1}-hybrids from reciprocal crosses of P. glauca and P. pungens, P. glauca and P. omorika, and F{sub 1}-hybrids of P. engelmannii x pungens. All 31 F{sub 1}-hybrids examined showed the cpDNA genotypes of the pollen parent, or the paternal species.

• Research Organization: Michigan State Univ., East Lansing, MI (USA)
• OSTI ID: 7194608
• Resource Relation: Other Information: Thesis (Ph.D)
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
SPRUCES
GENETICS
BIOLOGICAL MARKERS
CHLOROPLASTS
DNA
DNA-CLONING
ENDONUCLEASES
EXPERIMENTAL DATA
HYBRIDIZATION
MAIZE
PHOSPHORUS 32
POLLEN
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CELL CONSTITUENTS
CEREALS
CLONING
CONIFERS
DATA
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DNA-ASE
ENZYMES
ESTERASES
GAMETES
GERM CELLS
GRASS
HYDROLASES
INFORMATION
ISOTOPES
LIGHT NUCLEI
LILIOPSIDA
MAGNOLIOPHYTA
NUCLEI
NUCLEIC ACIDS
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS ISOTOPES
PINOPHYTA
PLANTS
RADIOISOTOPES
TREES
553002* - Agriculture & Food Technology- Horticulture- (1987-)