Mutation of Bacillus firmus OF4 to duramycin resistance result in substantial replacement of membrane lipid phosphatidylethanolamine by its plasmalogen form

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Journal of Bacteriology; (USA)

OSTI ID:7165214

Clejan, S ^[1]; Guffanti, A A; Cohen, M A; Krulwich, T A ^[2]

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1. City Hospital Center at Elmhurst, New York, NY (USA)
2. Medicine of the City Univ. of New York, NY (USA)

Mutant strains of alkalophilic Bacillus firms OF4 that were selected for resistance to duramycin had greatly reduced levels of membrane diacylphosphatidylethanolamine, as had been found in studies of such mutants of Bacillus subtilis. In the B. firmus strains, however, substantial levels of plasmenylethanolamine were found. This is an unusual membrane component for an aerobic eubacterium, but the presence of trace amounts even in the wild type was confirmed in experiments with {sup 32P}{sub i}-labeled growth medium. The membrane lipid composition of the duramycin-resistant strains had several other changes that also left alkalophilic growth unimpaired.

• DOE Contract Number: AC02-81ER10871
• OSTI ID: 7165214
• Journal Information: Journal of Bacteriology; (USA), Vol. 41:3; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIBIOTICS
BIOLOGICAL EFFECTS
BACILLUS
MEMBRANE PROTEINS
BIOSYNTHESIS
GROWTH
MUTATIONS
PHOSPHORUS 32
TRACER TECHNIQUES
ANTI-INFECTIVE AGENTS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques