Demonstration of the serum antibody to Epstein-Barr virus specific DNA polymerased (EBV-DP) from patients with nasopharyngeal carcinoma (NPC)
Raji cells, an EBV genome carrying and nonproducer cell line, treated with tetradecanoyl-phorbol-13-acetate (TPA) and n-butyrate could induce a special DNA polymerase which has properties that are similar to the EBV-DP induced by TPA in P/sub 3/HR-I cells, an EBV producer cell line. Since EBV was found to have a strong association with NPC, and antibodies against EBV proteins or enzymes were found in high levels in sera from these patients, the possible presence of serum antibody against EBV-DP was examined. The serum titer of antibody to EBV-DP was found to have 190 +/- 84 units/ml serum (mean +/- S.D.) in 48 sera from patients with NPC. The titer in 52 healthy donors was 31.4 +/- 28 unit/ml serum (p < 0.01). The antibody was found to be associated with the IgG but not the IgA fraction. The antibody titers against EBV-DP were not correlated with the titer against EBV-DNase or VCA-IgA. Whether the antibody observed is against an EBV-DP core protein or its stimulating protein, as demonstrated by this laboratory previously, is still being investigated. This study demonstrated the high frequency and high titer of antibody against EBV-DP in serum from patients with NPC, and suggested the potential of utilizing this antibody titer to compliment other methods for the early diagnosis or prognosis of NPC.
- Research Organization:
- Univ. of North Carolina, Chapel Hill
- OSTI ID:
- 7163914
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Studies on the induction of Epstein-Barr virus (EBV) DNA polymerase (POL) and deoxyribonuclease (DNase) by the combined action of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and N-butyrate (SB in EBV-carrying cells
Therapeutic targeting of regulatory T cells enhances tumor-specific CD8+ T cell responses in Epstein–Barr virus associated nasopharyngeal carcinoma
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CARCINOMAS
DIAGNOSIS
PHORBOL ESTERS
CARCINOGENESIS
ANTIBODIES
BUTYRIC ACID
DNA POLYMERASES
PATIENTS
PROTEINS
TOXICITY
CARBOXYLIC ACIDS
CARCINOGENS
DISEASES
ENZYMES
ESTERS
MONOCARBOXYLIC ACIDS
NEOPLASMS
NUCLEOTIDYLTRANSFERASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
TRANSFERASES
560300* - Chemicals Metabolism & Toxicology
550900 - Pathology