Expression and modulation of IL-1 alpha in murine keratinocytes
Murine and human keratinocytes produce an IL-1-like factor that appears to be similar if not identical to monocyte-derived IL-1. IL-1 may be an important mediator in cutaneous inflammatory responses, however, little is currently known concerning factors that may modulate IL-1 expression in keratinocytes. To address this issue we examined the effect of LPS, UV, and the cell differentiation state on murine keratinocyte IL-1 mRNA expression. Our results indicated that as with the murine P388D1 monocyte cell line, PAM 212 keratinocytes constitutively express abundant amounts of IL-1 alpha mRNA. On exposure to LPS (100 micrograms/ml) for 8 h there was more than 10 times the increase in PAM 212 IL-1 alpha mRNA which was accompanied by a sixfold increase in supernatant IL-1 activity. Similarly UV irradiation had a significant effect on keratinocyte IL-1 alpha expression. High dose UV (300 mJ/cm2) inhibited PAM 212 IL-1 alpha expression at 4, 8, 24, 48 h post-UV whereas a lower dose of UV (100 mJ/cm2) inhibited UV at 4 and 8 h post-UV, but induced IL-1 expression at 24 and 48 h post-UV. The expression of IL-1 alpha varied with the differentiation state of the keratinocytes. Freshly removed newborn murine keratinocytes were found to constitutively express IL-1 alpha mRNA. Keratinocytes grown in low (Ca2+) tissue culture media (0.05 mM) for 6 days, functionally and phenotypically become undifferentiated and express increased quantities of IL-1 alpha mRNA, whereas cells grown in high (Ca2+) media (1.2 mM) for 6 days become terminally differentiated and IL-1 expression ceased. Keratinocytes cultured for 3 days in low (Ca2+) conditions expressed an intermediate level of IL-1 alpha. In contrast, little or no IL-1 beta mRNA was detected in either the PAM 212 cells or newborn murine keratinocytes.
- Research Organization:
- National Cancer Institute, Bethesda, MD (USA)
- OSTI ID:
- 7130702
- Journal Information:
- J. Immunol.; (United States), Vol. 140:7
- Country of Publication:
- United States
- Language:
- English
Similar Records
Regulation of GM-CSF and IL-3 production from the murine keratinocyte cell line PAM 212 following exposure to ultraviolet radiation
Interleukin 1 gene expression in cultured human keratinocytes is augmented by ultraviolet irradiation
Related Subjects
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
CELL DIFFERENTIATION
GROWTH FACTORS
GENE REGULATION
CELL CULTURES
EPIDERMIS
LIPOPOLYSACCHARIDES
MESSENGER-RNA
MICE
ULTRAVIOLET RADIATION
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
ELECTROMAGNETIC RADIATION
EPITHELIUM
LIPIDS
MAMMALS
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RNA
RODENTS
SACCHARIDES
SKIN
TISSUES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture