Catabolic gene expression is monitored by bioluminescence in bioreactor studies
In order to study the expression of specific catabolic genes under defined conditions, and to determine whether certain conditions tend to increase or decrease metal catabolic activities, a bioreporter gene can be introduced into the microorganism. Activity from such bioreporter gene would indicate successful bioremediation. Our laboratory has produced several bioreporter strains using the bioluminescent lux genes of Vibrio fischeri. A bioreporter producing visible light when genetic expression is induced. The bioluminescent system include sensitivity of detection, analysis of response in real- time, and on-line capability. We constructed a bioreporter strain aimed at following the degradation of toluene and related compounds in order to study expression of the catabolic genes with various substrates and under optimized bioreactor conditions. We have been able to detect the induction of a specific operon in response to the addition of oxylene, as a gratuitous inducer of the catabolic genes. A strong bioluminescent signal in these studies. We have varied the medium of an induced bioreactor culture of RB1401, and our data suggest that conditions for optimal expression of the catabolic operon might not be identical with optimal growth conditions.
- Research Organization:
- Oak Ridge National Lab., TN (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 7096874
- Report Number(s):
- CONF-930482-1; ON: DE93006205
- Resource Relation:
- Conference: 2. international symposium in situ and on-site bioreclamation, San Diego, CA (United States), 5-8 Apr 1993
- Country of Publication:
- United States
- Language:
- English
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Regulated bioluminescence as a tool for bioremediation process monitoring and control of bacterial cultures
Regulated bioluminescence as a tool for bioremediation process monitoring and control of bacterial cultures
Related Subjects
BIOLUMINESCENCE
GENE REGULATION
PSEUDOMONAS
GENETIC ENGINEERING
TOLUENE
BIODEGRADATION
BIOREACTORS
GENE OPERONS
PLASMIDS
XYLENES
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIOTECHNOLOGY
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
HYDROCARBONS
LUMINESCENCE
MICROORGANISMS
ORGANIC COMPOUNDS
090900* - Biomass Fuels- Processing- (1990-)