Relationship between membrane potential changes and superoxide-releasing capacity in resident and activated mouse peritoneal macrophages

Journal Article · Fri Nov 01 00:00:00 EST 1985 · J. Immunol.; (United States)

OSTI ID:7093997

Kitagawa, S; Johnston, Jr, R B

To understand better the molecular basis for the enhanced respiratory burst of activated macrophages (M phi), the relationship between the stimulus-induced changes in membrane potential and release of superoxide anion (O/sub 2//sup -/) in mouse peritoneal M phi was investigated. Resident M phi and M phi elicited by injection of lipopolysaccharide (LPS-M phi) or obtained from animals infected with bacille Calmette-Guerin (BCG-M phi) were used. LPS-M phi and BCG-M phi showed more pronounced changes in membrane potential (depolarization) and greater release of O/sub 2//sup -/ on contact with phorbol myristate acetate (PMA) than did resident macrophages. The lag time between addition of stimulus and onset of release of O/sub 2//sup -/ was reduced in activated compared with resident cells. Membrane potential changes began 60 to 90 sec before release of O/sub 2//sup -/ could be detected in each cell type. The dose-response curves for triggering of membrane potential changes and O/sub 2//sup -/ release by PMA were identical. The magnitude of membrane potential changes and of O/sub 2//sup -/ release in LPS-M phi and BCG-M phi declined progressively during in vitro culture, and values on day 3 approached those in resident macrophages (deactivation). Extracellular glucose was required for effective stimulated change in membrane potential and O/sub 2//sup -/ release. These findings indicate that membrane potential changes are closely associated with O/sub 2//sup -/-releasing capacity in macrophages, and that the systems that mediate membrane potential changes and production of O/sub 2//sup -/ develop or decline concomitantly during activation or deactivation of the cells.

• Research Organization: National Jewish Hospital and Research Center, Denver, CO
• OSTI ID: 7093997
• Journal Information: J. Immunol.; (United States), Vol. 135:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
ELECTRIC POTENTIAL
MACROPHAGES
RESPIRATION
PHORBOL ESTERS
BIOLOGICAL EFFECTS
MICE
PERITONEUM
SUPEROXIDE RADICALS
ANIMAL CELLS
ANIMALS
CARCINOGENS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
ESTERS
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
PHAGOCYTES
RADICALS
RODENTS
SEROUS MEMBRANES
SOMATIC CELLS
VERTEBRATES
550200* - Biochemistry