Cytotoxic effects of benzene on mouse germ cells determined by flow cytometry

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Journal of Toxicology and Environmental Health; (USA)

DOI:https://doi.org/10.1080/15287398909531259· OSTI ID:7059439

Spano, M; Pacchierotti, F; Ucelli, R; Amendola, R; Bartoleschi, C ^[1]

---

1. ENEA, Casaccia, Rome (Italy)

Flow cytometric (FCM) DNA content measurements were performed on testicular monocellular suspensions obtained from mice exposed per os to 0, 1, 2, 4, 6, and 7 ml/kg body weight of benzene in order to investigate its cytotoxic action on gem cells. The effects of benzene were measured 7, 14, 21, 28, and 70 d after treatment. Benzene had no effect on testis weight, but FCM analysis showed the relative percentages of some cell subpopulations (tetraploid and haploid cells) to be different from the control pattern, indicating the occurrence of some cytotoxic damage to differentiating spermatogonia. These data demonstrate that spermatogenesis is sensitive to benzene single exposures as evidenced by an altered cell ratio of testicular cell types.

• OSTI ID: 7059439
• Journal Information: Journal of Toxicology and Environmental Health; (USA), Vol. 26:3; ISSN 0098-4108
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BENZENE
TOXICITY
SPERMATOGONIA
SENSITIVITY
CELL DIFFERENTIATION
CELL FLOW SYSTEMS
MICE
ORAL ADMINISTRATION
ANIMALS
AROMATICS
GAMETES
GERM CELLS
HYDROCARBONS
MAMMALS
ORGANIC COMPOUNDS
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology