Structure and function of the latent F sub 0 -F sub 1 -ATPase complex of Micrococcus lysodeikticus

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:7018896

Chung, Y S

The latent F{sub 0}F{sub 1}-ATPase from Micrococcus luteus (lysodeikticus) has been purified to homogeneity, and nine distinct subunit bands were observed on SDS-PAGE. Five of nine bands corresponded to the F{sub 1} subunits and the other four bands are likely to be subunits a, a{prime}, b, and c of the F{sub 0} segment of the complex. The subunit designated as a{prime} probably arises from proteolytic cleavage of the 25,5000 Mr subunit a. The F{sub 0}F{sub 1}-ATPase complex has a molecular weight of approximately 1,060,000, as determined by Fast Protein Liquid Chromatography (FPLC). It is assumed that the F{sub 0}F{sub 1}-ATPase peak obtained by FPLC was a dimer and that molecular weight of the F{sub 0}F{sub 1}-ATPase monomer was accordingly 530,000. The stoichiometry of the subunits was determined with {sup 14}C-labeled F{sub 0}F{sub 1}-ATPase prepared from cells grown on medium containing {sup 14}C-amino acids. Antibodies to the native and SDS-denatured F{sub 1} and F{sub 0}F{sub 1}-ATPase as well as to individual SDS-dissociated subunits have been generated for immunochemical analysis. The arrangement of the subunits in F{sub 1} and F{sub 0}F{sub 1}-ATPase have been investigated using bifunctional chemical cross-linking agents.

• Research Organization: New York Univ., NY (USA)
• OSTI ID: 7018896
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATP-ASE
BIOLOGICAL FUNCTIONS
MOLECULAR STRUCTURE
AMINO ACIDS
CARBON 14 COMPOUNDS
COMPLEXES
ELECTROPHORESIS
IMMUNOASSAY
LIQUID COLUMN CHROMATOGRAPHY
MICROCOCCUS LYSODEICTICUS
MOLECULAR WEIGHT
STOICHIOMETRY
TRACER TECHNIQUES
ACID ANHYDRASES
BACTERIA
BIOASSAY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ENZYMES
FUNCTIONS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROCOCCUS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
SEPARATION PROCESSES
550201* - Biochemistry- Tracer Techniques